[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00087_23H5VFLT4_s17.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15260733) splicing reads. 255237 splicing junctions. 179269 known splicing junctions. 75968 novel splicing junctions.
sampling 10% (30521467) splicing reads. 314569 splicing junctions. 192055 known splicing junctions. 122514 novel splicing junctions.
sampling 15% (45782200) splicing reads. 358497 splicing junctions. 199317 known splicing junctions. 159180 novel splicing junctions.
sampling 20% (61042934) splicing reads. 393376 splicing junctions. 204128 known splicing junctions. 189248 novel splicing junctions.
sampling 25% (76303668) splicing reads. 422491 splicing junctions. 207753 known splicing junctions. 214738 novel splicing junctions.
sampling 30% (91564401) splicing reads. 447173 splicing junctions. 210637 known splicing junctions. 236536 novel splicing junctions.
sampling 35% (106825135) splicing reads. 468795 splicing junctions. 212858 known splicing junctions. 255937 novel splicing junctions.
sampling 40% (122085869) splicing reads. 487587 splicing junctions. 214649 known splicing junctions. 272938 novel splicing junctions.
sampling 45% (137346602) splicing reads. 504292 splicing junctions. 216217 known splicing junctions. 288075 novel splicing junctions.
sampling 50% (152607336) splicing reads. 519083 splicing junctions. 217524 known splicing junctions. 301559 novel splicing junctions.
sampling 55% (167868070) splicing reads. 532061 splicing junctions. 218634 known splicing junctions. 313427 novel splicing junctions.
sampling 60% (183128803) splicing reads. 543672 splicing junctions. 219585 known splicing junctions. 324087 novel splicing junctions.
sampling 65% (198389537) splicing reads. 553849 splicing junctions. 220384 known splicing junctions. 333465 novel splicing junctions.
sampling 70% (213650271) splicing reads. 562883 splicing junctions. 221088 known splicing junctions. 341795 novel splicing junctions.
sampling 75% (228911004) splicing reads. 570766 splicing junctions. 221716 known splicing junctions. 349050 novel splicing junctions.
sampling 80% (244171738) splicing reads. 577656 splicing junctions. 222212 known splicing junctions. 355444 novel splicing junctions.
sampling 85% (259432472) splicing reads. 583540 splicing junctions. 222629 known splicing junctions. 360911 novel splicing junctions.
sampling 90% (274693205) splicing reads. 588597 splicing junctions. 222990 known splicing junctions. 365607 novel splicing junctions.
sampling 95% (289953939) splicing reads. 592726 splicing junctions. 223299 known splicing junctions. 369427 novel splicing junctions.
sampling 100% (305214673) splicing reads. 596099 splicing junctions. 223535 known splicing junctions. 372564 novel splicing junctions.