+ mkdir tih_rna_sample_00096_23H5VFLT4_s11 + all_qc -bam tih_rna_sample_00096_23H5VFLT4_s11.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder tih_rna_sample_00096_23H5VFLT4_s11 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai 0it [00:00, ?it/s] 75475it [00:00, 754702.14it/s][W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai 157955it [00:00, 795909.87it/s] 238786it [00:00, 801546.90it/s] 320898it [00:00, 809253.59it/s] 404065it [00:00, 817315.28it/s] 488117it [00:00, 825183.42it/s] 570636it [00:00, 821274.12it/s] 652768it [00:00, 818418.50it/s] 734614it [00:00, 815343.16it/s] 817000it [00:01, 817941.25it/s] 899460it [00:01, 819953.25it/s] 981459it [00:01, 816179.72it/s] 1063083it [00:01, 808278.51it/s] 1145783it [00:01, 813854.92it/s] 1227187it [00:01, 813266.52it/s] 1309523it [00:01, 816266.68it/s] 1391805it [00:01, 818212.10it/s] 1473635it [00:01, 816407.27it/s] 1555282it [00:01, 803974.78it/s] 1635723it [00:02, 803350.34it/s] 1719923it [00:02, 814797.10it/s] 1803202it [00:02, 820140.91it/s] 1886197it [00:02, 823054.37it/s] 1969778it [00:02, 826854.04it/s] 2053145it [00:02, 828876.01it/s] 2136044it [00:02, 820959.23it/s] 2220701it [00:02, 828565.17it/s] 2304170it [00:02, 830338.35it/s] 2387221it [00:02, 827578.64it/s] 2471186it [00:03, 831166.36it/s] 2554314it [00:03, 825926.53it/s] 2636921it [00:03, 825742.53it/s] 2719505it [00:03, 822984.14it/s] 2801811it [00:03, 821301.25it/s] 2883947it [00:03, 813625.31it/s] 2965393it [00:03, 813857.77it/s] 3048356it [00:03, 818538.49it/s] 3130860it [00:03, 820460.96it/s] 3215021it [00:03, 826760.34it/s] 3297706it [00:04, 826232.15it/s] 3380336it [00:04, 821251.19it/s] 3462784it [00:04, 822198.04it/s] 3545012it [00:04, 821457.40it/s] 3627163it [00:04, 817789.07it/s] 3708949it [00:04, 813423.96it/s] 3791467it [00:04, 816905.92it/s] 3874072it [00:04, 819617.05it/s] 3956041it [00:04, 818271.84it/s] 4037874it [00:04, 813961.23it/s] 4119278it [00:05, 812311.93it/s] 4200514it [00:05, 808271.41it/s] 4281347it [00:05, 805306.48it/s] 4361882it [00:05, 803009.70it/s] 4442412it [00:05, 803673.56it/s] 4523865it [00:05, 806839.32it/s] 4606478it [00:05, 812590.29it/s] 4689327it [00:05, 817326.68it/s] 4771065it [00:05, 810121.05it/s] 4852094it [00:05, 807607.78it/s] 4933089it [00:06, 808289.92it/s] 5013927it [00:06, 806179.27it/s] 5094552it [00:06, 801814.80it/s] 5175657it [00:06, 804450.66it/s] 5258242it [00:06, 810816.39it/s] 5341976it [00:06, 818726.59it/s] 5423858it [00:06, 816907.59it/s] 5507896it [00:06, 823855.70it/s] 5591341it [00:06, 826985.56it/s] 5674127it [00:06, 827229.94it/s] 5756854it [00:07, 825862.28it/s] 5839444it [00:07, 822026.48it/s] 5924691it [00:07, 831100.26it/s] 6008678it [00:07, 833706.07it/s] 6092056it [00:07, 832297.10it/s] 6175291it [00:07, 832221.75it/s] 6258517it [00:07, 826712.55it/s] 6341198it [00:07, 824553.07it/s] 6423661it [00:07, 818583.14it/s] 6505531it [00:07, 814124.48it/s] 6588153it [00:08, 817697.62it/s] 6670561it [00:08, 819579.32it/s] 6753225it [00:08, 821597.97it/s] 6838585it [00:08, 831147.97it/s] 6923000it [00:08, 835017.23it/s] 7006509it [00:08, 831531.89it/s] 7089670it [00:08, 831287.85it/s] 7172804it [00:08, 829793.86it/s] 7255788it [00:08, 827939.00it/s] 7338585it [00:08, 826323.24it/s] 7421781it [00:09, 827990.30it/s] 7504583it [00:09, 826186.49it/s] 7587459it [00:09, 826939.50it/s] 7672377it [00:09, 833454.02it/s] 7755725it [00:09, 824252.47it/s] 7838174it [00:09, 811849.66it/s] 7919479it [00:09, 812185.03it/s] 8002564it [00:09, 817699.29it/s] 8084365it [00:09, 811133.04it/s] 8165507it [00:09, 803837.55it/s] 8247347it [00:10, 808124.91it/s] 8329297it [00:10, 811485.00it/s] 8413125it [00:10, 819429.31it/s] 8495992it [00:10, 822167.79it/s] 8578879it [00:10, 824152.12it/s] 8661306it [00:10, 821996.63it/s] 8744245it [00:10, 824189.80it/s] 8826672it [00:10, 822216.10it/s] 8908900it [00:10, 817299.57it/s] 8992543it [00:10, 822982.08it/s] 9075322it [00:11, 824396.82it/s] 9158716it [00:11, 827107.29it/s] 9241433it [00:11, 825515.91it/s] 9323990it [00:11, 823389.89it/s] 9406333it [00:11, 814806.85it/s] 9487834it [00:11, 810520.30it/s] 9569882it [00:11, 813456.56it/s] 9651975it [00:11, 815613.45it/s] 9733911it [00:11, 816714.73it/s] 9817022it [00:11, 820999.59it/s] 9900490it [00:12, 825071.19it/s] 9983157it [00:12, 825464.12it/s] 10066890it [00:12, 829000.78it/s] 10149794it [00:12, 828760.50it/s] 10232673it [00:12, 827158.26it/s] 10315392it [00:12, 819863.61it/s] 10397622it [00:12, 820567.66it/s] 10481627it [00:12, 826363.72it/s] 10564390it [00:12, 826723.56it/s] 10647524it [00:12, 828085.56it/s] 10730339it [00:13, 824344.50it/s] 10812984it [00:13, 824957.10it/s] 10895486it [00:13, 822676.23it/s] 10977759it [00:13, 820583.72it/s] 11062994it [00:13, 830005.70it/s] 11148183it [00:13, 836530.71it/s] 11234066it [00:13, 843186.25it/s] 11320229it [00:13, 848692.17it/s] 11406656it [00:13, 853234.65it/s] 11492483it [00:13, 854725.77it/s] 11579498it [00:14, 859318.86it/s] 11667579it [00:14, 865744.70it/s] 11754317it [00:14, 866215.54it/s] 11840940it [00:14, 865016.44it/s] 11927443it [00:14, 863281.52it/s] 12013773it [00:14, 861063.37it/s] 12100347it [00:14, 862442.22it/s] 12186594it [00:14, 861587.61it/s] 12272755it [00:14, 859730.81it/s] 12358730it [00:14, 858953.50it/s] 12444627it [00:15, 858262.09it/s] 12531027it [00:15, 859960.88it/s] 12617024it [00:15, 859293.91it/s] 12704111it [00:15, 862736.66it/s] 12790405it [00:15, 862776.26it/s] 12876684it [00:15, 859686.25it/s] 12963154it [00:15, 861153.94it/s] 13049272it [00:15, 858480.38it/s] 13137275it [00:15, 864898.48it/s] 13224154it [00:16, 866043.95it/s] 13311641it [00:16, 868666.60it/s] 13398511it [00:16, 862838.33it/s] 13484806it [00:16, 859451.27it/s] 13570760it [00:16, 831874.64it/s] 13654130it [00:16, 824971.17it/s] 13736751it [00:16, 823599.91it/s] 13820533it [00:16, 827740.82it/s] 13904210it [00:16, 830395.05it/s] 13987300it [00:16, 825975.87it/s] 14069935it [00:17, 821172.15it/s] 14152080it [00:17, 820728.52it/s] 14234172it [00:17, 817767.43it/s] 14315962it [00:17, 814609.86it/s] 14397433it [00:17, 810677.50it/s] 14478509it [00:17, 803591.34it/s] 14558882it [00:17, 795175.48it/s] 14638419it [00:17, 790868.61it/s] 14718783it [00:17, 794624.69it/s] 14799341it [00:17, 797756.35it/s] 14880304it [00:18, 801274.21it/s] 14960444it [00:18, 800585.83it/s] 15040511it [00:18, 798660.49it/s] 15120383it [00:18, 794241.01it/s] 15199815it [00:18, 793267.21it/s] 15279147it [00:18, 792501.10it/s] 15359973it [00:18, 797186.10it/s] 15440659it [00:18, 800048.31it/s] 15520668it [00:18, 798494.23it/s] 15600521it [00:18, 795792.76it/s] 15680104it [00:19, 794281.68it/s] 15759535it [00:19, 792992.83it/s] 15838836it [00:19, 792097.86it/s] 15918047it [00:19, 789692.88it/s] 15998023it [00:19, 792611.87it/s] 16077448it [00:19, 793080.93it/s] 16157408it [00:19, 794934.57it/s] 16236904it [00:19, 793076.65it/s] 16316214it [00:19, 791977.93it/s] 16395651it [00:19, 792674.40it/s] 16475114it [00:20, 793247.61it/s][W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai 16554440it [00:20, 793190.93it/s] 16633760it [00:20, 788290.58it/s] 16712596it [00:20, 778841.44it/s] 16790507it [00:20, 778255.24it/s] 16870519it [00:20, 784736.78it/s] 16949585it [00:20, 786485.35it/s] 17030428it [00:20, 793020.34it/s] 17111100it [00:20, 797098.77it/s] 17190925it [00:20, 797328.08it/s] 17270665it [00:21, 795635.77it/s] 17350235it [00:21, 795465.12it/s] 17430384it [00:21, 797249.79it/s] 17511021it [00:21, 799865.02it/s] 17592099it [00:21, 803116.02it/s] 17672413it [00:21, 800427.91it/s] 17752460it [00:21, 797589.80it/s] 17832376it [00:21, 798039.06it/s] 17912183it [00:21, 796028.64it/s] 17992400it [00:21, 797845.71it/s] 18072606it [00:22, 799088.41it/s] 18091183it [00:22, 819346.28it/s] [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai [W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00096_23H5VFLT4_s11.md.bai + merge_qc -folders tih_rna_sample_00096_23H5VFLT4_s11/tih_rna_sample_00096_23H5VFLT4_s11.md -output tih_rna_sample_00096_23H5VFLT4_s11/tih_rna_sample_00096_23H5VFLT4_s11_full_basic_stats.tsv + head -n1 tih_rna_sample_00096_23H5VFLT4_s11/tih_rna_sample_00096_23H5VFLT4_s11_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed tih_rna_sample_00096_23H5VFLT4_s11/tih_rna_sample_00096_23H5VFLT4_s11_full_basic_stats.tsv