[W::hts_idx_load3] The index file is older than the data file: tih_rna_sample_00097_23H5VFLT4_s12.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (16178735) splicing reads. 273913 splicing junctions. 185975 known splicing junctions. 87938 novel splicing junctions.
sampling 10% (32357470) splicing reads. 344407 splicing junctions. 199508 known splicing junctions. 144899 novel splicing junctions.
sampling 15% (48536205) splicing reads. 395815 splicing junctions. 207067 known splicing junctions. 188748 novel splicing junctions.
sampling 20% (64714940) splicing reads. 436342 splicing junctions. 211940 known splicing junctions. 224402 novel splicing junctions.
sampling 25% (80893676) splicing reads. 469824 splicing junctions. 215461 known splicing junctions. 254363 novel splicing junctions.
sampling 30% (97072411) splicing reads. 498285 splicing junctions. 218176 known splicing junctions. 280109 novel splicing junctions.
sampling 35% (113251146) splicing reads. 522353 splicing junctions. 220370 known splicing junctions. 301983 novel splicing junctions.
sampling 40% (129429881) splicing reads. 543389 splicing junctions. 222136 known splicing junctions. 321253 novel splicing junctions.
sampling 45% (145608616) splicing reads. 561616 splicing junctions. 223561 known splicing junctions. 338055 novel splicing junctions.
sampling 50% (161787352) splicing reads. 577552 splicing junctions. 224763 known splicing junctions. 352789 novel splicing junctions.
sampling 55% (177966087) splicing reads. 591598 splicing junctions. 225782 known splicing junctions. 365816 novel splicing junctions.
sampling 60% (194144822) splicing reads. 603944 splicing junctions. 226626 known splicing junctions. 377318 novel splicing junctions.
sampling 65% (210323557) splicing reads. 614618 splicing junctions. 227342 known splicing junctions. 387276 novel splicing junctions.
sampling 70% (226502292) splicing reads. 623704 splicing junctions. 227947 known splicing junctions. 395757 novel splicing junctions.
sampling 75% (242681028) splicing reads. 631658 splicing junctions. 228458 known splicing junctions. 403200 novel splicing junctions.
sampling 80% (258859763) splicing reads. 638417 splicing junctions. 228862 known splicing junctions. 409555 novel splicing junctions.
sampling 85% (275038498) splicing reads. 644036 splicing junctions. 229226 known splicing junctions. 414810 novel splicing junctions.
sampling 90% (291217233) splicing reads. 648488 splicing junctions. 229528 known splicing junctions. 418960 novel splicing junctions.
sampling 95% (307395968) splicing reads. 651938 splicing junctions. 229745 known splicing junctions. 422193 novel splicing junctions.
sampling 100% (323574704) splicing reads. 654293 splicing junctions. 229887 known splicing junctions. 424406 novel splicing junctions.