FastQCFastQC Report
Sat 16 May 2026
NTC_0001_0001_23LGNLLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_23LGNLLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641054
Total Bases96.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGG43425167.74015917535809TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGT8070712.589735030122268TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTAGG133052.075488180402899TruSeq Adapter, Index 23 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGGG75391.1760319723455435TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTAGT51060.7965007628062534TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCGGGG29080.45362793150031044TruSeq Adapter, Index 23 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGGGTG24310.37921922334155933TruSeq Adapter, Index 23 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGGGGG19180.2991947636236573TruSeq Adapter, Index 23 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATGTGGG16800.2620684060937144TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATTTGGG16800.2620684060937144TruSeq Adapter, Index 23 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGGA12510.19514736668049806TruSeq Adapter, Index 23 (97% over 37bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGG12130.1892196289236164TruSeq Adapter, Index 6 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA9140.14257769236288986No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGTG8700.1357139960128164TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCTGGGG7310.11403095527053883TruSeq Adapter, Index 6 (96% over 30bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC6800.10607530722840822No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATCGGGT6510.10155150736131434TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCACTACCAGATATGGG6450.1006155487681225TruSeq Adapter, Index 23 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)