FastQCFastQC Report
Wed 4 Mar 2026
FFPE_HD834_01_RNA_0001_23LGNLLT4_3_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameFFPE_HD834_01_RNA_0001_23LGNLLT4_3_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000000
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCAGGCTTATCTAGT1201720.12017200000000001TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)