File Info

Filename
qc_report_detailed.txt
Full Path
s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0002_exp0005/A23LGNLLT4/nfcore_rnafusion__73d51683--20260607-225254/samplesheet_check/qc_report_detailed.txt
Size
11.7 KB
Published
Jun 07, 2026 11:50 PM
================================================================================
BCL CONVERT QC REPORT - DETAILED
================================================================================

1. % UNDETERMINED PER LANE
--------------------------------------------------------------------------------
      %_Undetermined  Undetermined_Reads  Total_Reads
Lane                                                 
1               5.22           211346066   4046804284
2               5.33           213604774   4005741146
3               5.18           210105409   4055602240
4               4.90           196957742   4015754163
5               3.56           144447804   4059237853
6               3.75           152658631   4075260777
7               3.85           158180325   4111566941
8               3.87           157368459   4070182884

2. SAMPLES WITH LOW READS (NON-NTC)
--------------------------------------------------------------------------------
No samples found with low reads

3. NTC SAMPLES WITH HIGH READS
--------------------------------------------------------------------------------
Found 1 NTC samples with high reads:
                    SampleID                  Index  # Reads
0  NTC_0001_0001_23LGNLLT4_1  AATTGGCACA-CAGTCGGAGT  3344443

4. TOP UNKNOWN BARCODES
--------------------------------------------------------------------------------
Found 81 significant unknown barcodes:
    Lane       index      index2   # Reads  % of Unknown Barcodes  % of All Reads  Percentage_of_Lane  Percentage_of_Total
0      1  CCAACTGTAT  GGGGGGGGGG  16512354               0.078129        0.004080            0.408034             0.050901
1      1  GTGACGCACA  GGGGGGGGGG   6109919               0.028910        0.001510            0.150981             0.018834
2      1  GAGTAGTGAC  GGGGGGGGGG   5095319               0.024109        0.001259            0.125910             0.015707
3      1  GATAGGTGAA  GGGGGGGGGG   4626452               0.021890        0.001143            0.114324             0.014261
4      1  AGACGACAGA  GGGGGGGGGG   4425791               0.020941        0.001094            0.109365             0.013643
5      1  TGAGTGTACA  GGGGGGGGGG   3787896               0.017923        0.000936            0.093602             0.011677
6      1  TAGCGAGGCT  GGGGGGGGGG   3727400               0.017636        0.000921            0.092107             0.011490
7      1  AAGGTGTACT  GGGGGGGGGG   3677456               0.017400        0.000909            0.090873             0.011336
8      1  CTCTATACTG  GGGGGGGGGG   3616335               0.017111        0.000894            0.089363             0.011148
9      1  TGTGACGTAT  GGGGGGGGGG   3000754               0.014198        0.000742            0.074151             0.009250
10     1  ACCAGGAATT  GGGGGGGGGG   2549661               0.012064        0.000630            0.063004             0.007860
11     1  TAAGGCTCAC  GGGGGGGGGG   2174868               0.010291        0.000537            0.053743             0.006704
12     1  ATCATACCGC  GGGGGGGGGG   2147851               0.010163        0.000531            0.053075             0.006621
13     1  TCTCCGTACT  GGGGGGGGGG   2053900               0.009718        0.000508            0.050754             0.006331
14     2  CCAACTGTAT  GGGGGGGGGG  15161678               0.070980        0.003785            0.378499             0.046737
15     2  GTGACGCACA  GGGGGGGGGG   5669869               0.026544        0.001415            0.141544             0.017478
16     2  GAGTAGTGAC  GGGGGGGGGG   5064184               0.023708        0.001264            0.126423             0.015611
17     2  GATAGGTGAA  GGGGGGGGGG   4188170               0.019607        0.001046            0.104554             0.012910
18     2  AGACGACAGA  GGGGGGGGGG   4035783               0.018894        0.001007            0.100750             0.012441
19     2  TAGCGAGGCT  GGGGGGGGGG   3606840               0.016886        0.000900            0.090042             0.011118
20     2  TGAGTGTACA  GGGGGGGGGG   3514278               0.016452        0.000877            0.087731             0.010833
21     2  AAGGTGTACT  GGGGGGGGGG   3471119               0.016250        0.000867            0.086654             0.010700
22     2  CTCTATACTG  GGGGGGGGGG   3427535               0.016046        0.000856            0.085566             0.010566
23     2  ATCCAGCATG  AGATCTCGGT   3346817               0.015668        0.000836            0.083551             0.010317
24     2  TGTGACGTAT  GGGGGGGGGG   2561274               0.011991        0.000639            0.063940             0.007895
25     2  ACCAGGAATT  GGGGGGGGGG   2473783               0.011581        0.000618            0.061756             0.007626
26     2  ATCATACCGC  GGGGGGGGGG   2088118               0.009776        0.000521            0.052128             0.006437
27     2  TAAGGCTCAC  GGGGGGGGGG   2028720               0.009498        0.000506            0.050645             0.006254
28     3  CCAACTGTAT  GGGGGGGGGG  15100371               0.071870        0.003723            0.372334             0.046548
29     3  GTGACGCACA  GGGGGGGGGG   5596458               0.026636        0.001380            0.137993             0.017252
30     3  GAGTAGTGAC  GGGGGGGGGG   4917015               0.023403        0.001212            0.121240             0.015157
31     3  GATAGGTGAA  GGGGGGGGGG   4171417               0.019854        0.001029            0.102856             0.012859
32     3  AGACGACAGA  GGGGGGGGGG   3959603               0.018846        0.000976            0.097633             0.012206
33     3  TGAGTGTACA  GGGGGGGGGG   3470378               0.016517        0.000856            0.085570             0.010698
34     3  TAGCGAGGCT  GGGGGGGGGG   3457160               0.016454        0.000852            0.085244             0.010657
35     3  AAGGTGTACT  GGGGGGGGGG   3367739               0.016029        0.000830            0.083039             0.010381
36     3  CTCTATACTG  GGGGGGGGGG   3290240               0.015660        0.000811            0.081128             0.010142
37     3  ATCCAGCATG  AGATCTCGGT   3113478               0.014819        0.000768            0.076770             0.009598
38     3  TGTGACGTAT  GGGGGGGGGG   2514623               0.011968        0.000620            0.062004             0.007752
39     3  ACCAGGAATT  GGGGGGGGGG   2425246               0.011543        0.000598            0.059800             0.007476
40     4  CCAACTGTAT  GGGGGGGGGG  14837857               0.075335        0.003695            0.369491             0.045739
41     4  GTGACGCACA  GGGGGGGGGG   5492973               0.027889        0.001368            0.136786             0.016933
42     4  GAGTAGTGAC  GGGGGGGGGG   4397564               0.022327        0.001095            0.109508             0.013556
43     4  GATAGGTGAA  GGGGGGGGGG   4211227               0.021381        0.001049            0.104868             0.012982
44     4  AGACGACAGA  GGGGGGGGGG   3868534               0.019641        0.000963            0.096334             0.011925
45     4  TGAGTGTACA  GGGGGGGGGG   3474004               0.017638        0.000865            0.086509             0.010709
46     4  TAGCGAGGCT  GGGGGGGGGG   3315912               0.016836        0.000826            0.082573             0.010222
47     4  AAGGTGTACT  GGGGGGGGGG   3173047               0.016110        0.000790            0.079015             0.009781
48     4  CTCTATACTG  GGGGGGGGGG   3114019               0.015811        0.000775            0.077545             0.009599
49     4  TGTGACGTAT  GGGGGGGGGG   2835605               0.014397        0.000706            0.070612             0.008741
50     4  ACCAGGAATT  GGGGGGGGGG   2230348               0.011324        0.000555            0.055540             0.006875
51     4  TAAGGCTCAC  GGGGGGGGGG   2041112               0.010363        0.000508            0.050828             0.006292
52     5  TTGGATTGTC  GGGGGGGGGG   6386285               0.044212        0.001573            0.157327             0.019686
53     5  TGCAACACTA  GGGGGGGGGG   3653641               0.025294        0.000900            0.090008             0.011263
54     5  TCGCATGACA  GGGGGGGGGG   3506075               0.024272        0.000864            0.086373             0.010808
55     5  CATCAGGCGT  GGGGGGGGGG   3140590               0.021742        0.000774            0.077369             0.009681
56     5  CCACCTGCTT  GGGGGGGGGG   3040546               0.021049        0.000749            0.074904             0.009373
57     5  CCAGTGCACT  GGGGGGGGGG   2445200               0.016928        0.000602            0.060238             0.007538
58     6  TTGGATTGTC  GGGGGGGGGG   6897136               0.045180        0.001692            0.169244             0.021261
59     6  TGCAACACTA  GGGGGGGGGG   3977480               0.026055        0.000976            0.097601             0.012261
60     6  TCGCATGACA  GGGGGGGGGG   3856686               0.025263        0.000946            0.094637             0.011889
61     6  CATCAGGCGT  GGGGGGGGGG   3410098               0.022338        0.000837            0.083678             0.010512
62     6  CCACCTGCTT  GGGGGGGGGG   3267007               0.021401        0.000802            0.080167             0.010071
63     6  CCAGTGCACT  GGGGGGGGGG   2652819               0.017377        0.000651            0.065096             0.008178
64     7  TTGGATTGTC  GGGGGGGGGG   7196004               0.045492        0.001750            0.175019             0.022182
65     7  TGCAACACTA  GGGGGGGGGG   4140080               0.026173        0.001007            0.100693             0.012762
66     7  TCGCATGACA  GGGGGGGGGG   4133143               0.026129        0.001005            0.100525             0.012741
67     7  CATCAGGCGT  GGGGGGGGGG   3609071               0.022816        0.000878            0.087778             0.011125
68     7  CCACCTGCTT  GGGGGGGGGG   3599203               0.022754        0.000875            0.087538             0.011095
69     7  CCAGTGCACT  GGGGGGGGGG   2744600               0.017351        0.000668            0.066753             0.008461
70     7  GAAGCAAGCG  GGGGGGGGGG   2170074               0.013719        0.000528            0.052780             0.006689
71     7  ACAATAGCAC  GGGGGGGGGG   2116078               0.013378        0.000515            0.051466             0.006523
72     8  TTGGATTGTC  GGGGGGGGGG   7535038               0.047882        0.001851            0.185128             0.023228
73     8  TGCAACACTA  GGGGGGGGGG   4232194               0.026894        0.001040            0.103980             0.013046
74     8  TCGCATGACA  GGGGGGGGGG   4042491               0.025688        0.000993            0.099320             0.012461
75     8  CATCAGGCGT  GGGGGGGGGG   3662512               0.023273        0.000900            0.089984             0.011290
76     8  CCACCTGCTT  GGGGGGGGGG   3431616               0.021806        0.000843            0.084311             0.010578
77     8  CCAGTGCACT  GGGGGGGGGG   2926417               0.018596        0.000719            0.071899             0.009021
78     8  GAAGCAAGCG  GGGGGGGGGG   2275317               0.014459        0.000559            0.055902             0.007014
79     8  ACAATAGCAC  GGGGGGGGGG   2140678               0.013603        0.000526            0.052594             0.006599
80     8  CGTACTTAGA  GGGGGGGGGG   2057009               0.013071        0.000505            0.050538             0.006341

5. BARCODES WITH HIGH MISMATCH RATES
--------------------------------------------------------------------------------
No barcodes found with high mismatch rates

================================================================================
FINAL STATUS CHECK
================================================================================
Overall % Undetermined: 4.45%
Status: FAIL
Reason(s):
  NTC sample NTC_0001_0001_23LGNLLT4_1 has 3,344,443 reads > 2,000,000