[W::hts_idx_load3] The index file is older than the data file: V4_0001_RNA_0005_23H5VFLT4_s20.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	286176859
Known Splicing Events:	282964665
Partial Novel Splicing Events:	1954544
Novel Splicing Events:	1158844
Filtered Splicing Events:	98806

Total splicing  Junctions:	476907
Known Splicing Junctions:	241883
Partial Novel Splicing Junctions:	142130
Novel Splicing Junctions:	92894

===================================================================
Create BED file ...
Create Interact file ...