STAR-FUSION FILTERING SUMMARY ================================================================================ Input file: FFPE_HD835_01_RNA_0001_23LGNLLT4_3.starfusion.starfusion.fusion_predictions.tsv Blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz Filter config: starfusion_filter_config.yaml Output file: FFPE_HD835_01_RNA_0001_23LGNLLT4_3.starfusion.starfusion.fusion_predictions.filter_summary.tsv Total fusions: 1796 Pass (CALL=1): 11 (0.6%) Fail (CALL=0): 1785 (99.4%) Blacklist Flagging: Total blacklisted: 295 All blacklisted fusions have CALL=0 Failure Reasons: Read count too low: 1 < 8: 954 Excluded SpliceType: INCL_NON_REF_SPLICE: 432 Blacklisted: 172 Blacklisted; aih_curated_blacklist score(3): 85 Read count too low: 2 < 8: 36 aih_curated_blacklist score(3): 35 Excluded annotation pattern: HGNC_GENEFAM: 19 Read count too low: 3 < 8: 9 Excluded annotation pattern: GTEx_recurrent_StarF2019: 7 Read count too low: 4 < 8: 6 Excluded annotation pattern: DGD_PARALOGS: 6 Does not meet inclusion criteria: 5 Read count too low: 5 < 8: 5 Excluded annotation pattern: ConjoinG: 5 Read count too low: 6 < 8: 4 aih_curated_blacklist score(5): 2 Read count too low: 7 < 8: 2 aih_curated_blacklist score(6): 1 Breakdown by SpliceType: INCL_NON_REF_SPLICE: 0 pass, 804 fail (total: 804) ONLY_REF_SPLICE: 11 pass, 981 fail (total: 992)