FastQCFastQC Report
Wed 4 Mar 2026
NTC_0001_0001_23KKJ7LT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_23KKJ7LT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189071
Total Bases934.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGT213016234.41812187968114TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGG135337621.867191376541005TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGG98140515.857064816351274TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGT6035019.751075726874033TruSeq Adapter, Index 13 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGTGTG532210.8599190411614279TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGTATG500440.8085866198658894TruSeq Adapter, Index 13 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAG308830.49899249822792463TruSeq Adapter, Index 13 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGGGTG287140.4639468508278544TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGTGTG271010.43788478109234813TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGGGGG265630.4291920386759176TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGGGTG229430.3707018387735413TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGGGGG221050.3571618422215547TruSeq Adapter, Index 13 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGG199850.3229079129969587TruSeq Adapter, Index 13 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGGATG122050.1972024557482052TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGTATG115110.18598914118128554TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGGG110160.17799117185761804TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTGGGG102580.16574377640844645TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGTA84720.13688645678810277TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAATCGCATCTAGTG80460.13000335591561318TruSeq Adapter, Index 13 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA73210.11828915842135274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)