FastQCFastQC Report
Thu 26 Feb 2026
22Rv1_FFPE_RNA_0001_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22Rv1_FFPE_RNA_0001_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15751549
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTCTGAATCTGGG9204925.843818915841229TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTCTGAATCTGGT8911085.657272183199252TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTCTGAATCTAGT1896371.2039260392739786TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTCTGAATCTAGG1445780.9178652842333157TruSeq Adapter, Index 1 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTCTGAATCTGGGG211240.13410744555979862TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)