FastQCFastQC Report
Thu 26 Feb 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980467
Total Bases450 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGT170008357.04082615241168TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGT73206924.56222464466139TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGG514091.7248639223316347TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGG324161.0876147932521985TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA304541.0217861831719661TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTATG194900.6539243682282004TruSeq Adapter, Index 7 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGTA142440.47791168296780334TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGTATG83070.2787147114864885TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCGAGT54760.18372959673769246TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCGGGT44150.14813114857503876TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATGTAGT42260.14178986044804387TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATTTAGT40430.13564988305523934TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATTTGGT39440.13232825594109915TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCANTCACAAGGCTCCATATCTAGT37510.12585276065797743TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATGTGGT37270.12504751772121617TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGTG37190.12477910340896242TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACNTCTGAACTCCAGTCACAAGGCTCCATATCTAGT37120.1245442408857404TruSeq Adapter, Index 1 (97% over 34bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA30600.1026684744370597No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)