Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44330309036(97.8594%) Q30 bases: 42708647498(94.2796%) Read2 before filtering: total reads: 300000000 total bases: 45300000000 Q20 bases: 44288365519(97.7668%) Q30 bases: 42269524432(93.3102%) Read1 after filtering: total reads: 294875022 total bases: 37302930763 Q20 bases: 37109893660(99.4825%) Q30 bases: 36291036957(97.2874%) Read2 after filtering: total reads: 294875022 total bases: 37348802207 Q20 bases: 37040590030(99.1748%) Q30 bases: 36009875968(96.4151%) Filtering result: reads passed filter: 589750044 reads failed due to low quality: 8344772 reads failed due to too many N: 191748 reads failed due to too short: 1713436 reads with adapter trimmed: 362919022 bases trimmed due to adapters: 14564494059 Duplication rate: 25.6357% Insert size peak (evaluated by paired-end reads): 118 JSON report: SW780_FFPE_RNA_0001_B23LG7FLT4_1.fastp.json HTML report: SW780_FFPE_RNA_0001_B23LG7FLT4_1.fastp.html fastp --in1 SW780_FFPE_RNA_0001_B23LG7FLT4_1_1.fastq.gz --in2 SW780_FFPE_RNA_0001_B23LG7FLT4_1_2.fastq.gz --out1 SW780_FFPE_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz --out2 SW780_FFPE_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz --json SW780_FFPE_RNA_0001_B23LG7FLT4_1.fastp.json --html SW780_FFPE_RNA_0001_B23LG7FLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 810 seconds