FastQCFastQC Report
Sat 16 May 2026
ColoN_FFPE_L06_RNA_01_B23LG7FLT4_2_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameColoN_FFPE_L06_RNA_01_B23LG7FLT4_2_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132597913
Total Bases20 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAAGATCTGGT50106403.778822672721855TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAAGATCTGGG32879212.4796174582325436TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAAGATCTAGT6748050.5089107247110292TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAACAAGATCTAGG3276010.24706346622514336TruSeq Adapter, Index 9 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)