FastQCFastQC Report
Tue 3 Mar 2026
NTC_0001_0001_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874575
Total Bases132 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGT53735661.441957522225074TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTGGT17261219.736672097876113TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATATATCTAGT121941.394277220364177TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA97431.1140268130234685TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCATATATCTGGT68010.7776348512134466TruSeq Adapter, Index 13 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTATG64640.7391018494697424TruSeq Adapter, Index 13 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTGGG39620.45302003830431925TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTGGTA35720.40842695023297027TruSeq Adapter, Index 13 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTGGTATG21800.24926392819369408TruSeq Adapter, Index 13 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATTTAGT18670.21347511648514994TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCGAGT17910.20478518137381016TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATTTGGT16180.1850041448703656TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATGTAGT15570.17802932853100076TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGG15260.17448474973558586TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATGTGGT13560.15504673698653632TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCGGGT12480.1426978818283166TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCANTCACCGTCGCCTATATCTAGT11860.13560872423748677TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAAGAGCACACNTCTGAACTCCAGTCACCGTCGCCTATATCTAGT11650.13320755795672182TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA10580.12097304404996713TruSeq Adapter, Index 22 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)