Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 874575 total bases: 132060825 Q20 bases: 122880494(93.0484%) Q30 bases: 105032897(79.5337%) Read2 before filtering: total reads: 874575 total bases: 132060825 Q20 bases: 113467941(85.921%) Q30 bases: 77415985(58.6215%) Read1 after filtering: total reads: 45983 total bases: 2684648 Q20 bases: 2592932(96.5837%) Q30 bases: 2400520(89.4166%) Read2 after filtering: total reads: 45983 total bases: 2768523 Q20 bases: 2667128(96.3376%) Q30 bases: 2436900(88.0217%) Filtering result: reads passed filter: 91966 reads failed due to low quality: 137382 reads failed due to too many N: 38 reads failed due to too short: 1519764 reads with adapter trimmed: 905512 bases trimmed due to adapters: 129998903 Duplication rate: 53.3761% Insert size peak (evaluated by paired-end reads): 31 JSON report: NTC_0001_0001_B23LG7FLT4_1.fastp.json HTML report: NTC_0001_0001_B23LG7FLT4_1.fastp.html fastp --in1 NTC_0001_0001_B23LG7FLT4_1_1.fastq.gz --in2 NTC_0001_0001_B23LG7FLT4_1_2.fastq.gz --out1 NTC_0001_0001_B23LG7FLT4_1_1.fastp.fastq.gz --out2 NTC_0001_0001_B23LG7FLT4_1_2.fastp.fastq.gz --json NTC_0001_0001_B23LG7FLT4_1.fastp.json --html NTC_0001_0001_B23LG7FLT4_1.fastp.html --thread 12 --detect_adapter_for_pe fastp v0.23.4, time used: 5 seconds