FastQCFastQC Report
Tue 3 Mar 2026
NTC_0001_0001_B23LG7FLT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23LG7FLT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45983
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC75

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG500510.884457299436749No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC6971.5157775699715113No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6371.3852945653828588No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2920.6350172889981079No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2810.6110954048235218No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC2420.5262814518408977No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC2270.49366070069373463No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1890.41102146445425486No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG1770.38492486353652433No Hit
TTCCCCACCGGGCCTTCCCGT1720.3740512798208034No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1390.30228562729704456No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1250.27183959289302567No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG1210.26314072592044885No Hit
TTCCCCACCGGGCCTTCCCGTG1200.2609660091773046No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1200.2609660091773046No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC1180.25661657569101626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGG1080.2348694082595742No Hit
GTGCTGCTGCCGGGGGGGC990.21529695757127632No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC950.20659809059869952No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG860.1870256399104017No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCCGCG840.18267620642411325No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG820.17832677293782484No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC820.17832677293782484No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC820.17832677293782484No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCC780.16962790596524802No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC750.1631037557358154No Hit
TTCCCCACCGGGCCTTCCCCC710.1544048887632386No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC700.15223017202009437No Hit
TTCCCCACCGGGCCTTCCCCG690.15005545527695016No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC690.15005545527695016No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG690.15005545527695016No Hit
CTTCGCGCCCCCCGTTCCTCC670.14570602179066178No Hit
GTGCTGCTGCCGGGGGGGCC650.14135658830437334No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC640.13918187156122913No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC630.13700715481808495No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG600.1304830045886523No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC590.12830828784550813No Hit
GCCCCCCGGCCCCGCGTCC550.1196094208729313No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG530.11525998738664289No Hit
GACTGGAGTTCAGACGTGTGCTCTCCGATC510.11091055390035447Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGCTGGGTCGGTCGGGCTGG500.10873583715721027No Hit
GCTGCTGCCGGGGGGGC490.10656112041406607No Hit
GGCCCCCCCGGCAGCAGC490.10656112041406607No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGG490.10656112041406607No Hit
GCTGCTGCCGGGGGGGCC480.10438640367092186No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG480.10438640367092186No Hit
CGCCCTTCTTTCTCTTTCCCTCCCCCGCCTCCTCG480.10438640367092186No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG460.10003697018463346No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCATTCGTAT460.10003697018463346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)