FastQCFastQC Report
Tue 3 Mar 2026
NTC_0001_0001_B23LG7FLT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23LG7FLT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45983
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA9932.159493725942196TruSeq Adapter, Index 22 (96% over 31bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6251.3591979644651284No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC4120.8959832981754127No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT3450.750277276384751No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2970.645890872713829No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTGGTA2800.6089206880803775TruSeq Adapter, Index 22 (96% over 31bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC2770.6023965378509449No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG2690.5849988039057913No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA2410.5241067350977535No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA2320.5045342844094557No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC1890.41102146445425486No Hit
ACGGGAAGGCCCGGTGGGGAAA1700.3697018463345149No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACCGTCGCCTATATCTAGTA1620.3523041123893613Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGTCGCCTATATCTAGTA1500.3262075114716308Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC1400.30446034404018874No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA1360.29576147706761197No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1320.28706261009503514No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAG1300.2827131766087467TruSeq Adapter, Index 22 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCGTCGCCTATATCTAG1240.2696648761498815Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC1220.2653154426635931No Hit
CACGGGAAGGCCCGGTGGGGAAA1200.2609660091773046No Hit
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1150.2500924254615836No Hit
GATCGGAAGAGCACACGTCTGAACCCAGTCACCGTCGCCTATATCTAGTA1130.24574299197529523Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACCGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA1100.2392188417458626TruSeq Adapter, Index 13 (95% over 22bp)
GCCCCCCCGGCAGCAGCACA1000.21747167431442055No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGG980.21312224082813214No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA970.21094752408498796TruSeq Adapter, Index 22 (95% over 23bp)
GATCGGAAGAGCACACGTTCTGAACTCCAGTCACCGTCGCCTATATCTAG960.20877280734184372No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA950.20659809059869952TruSeq Adapter, Index 22 (95% over 21bp)
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC890.19354979013983428No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACCGTCGCCTATATCTAGTA870.18920035665354587Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTTGAACTCCAGTCACCGTCGCCTATATCTAG860.1870256399104017No Hit
GATCGGAAGAGCACACGTCTGAACTTCCAGTCACCGTCGCCTATATCTAG840.18267620642411325Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCGGGGGAGAGAAAGAGAAAGAAGGGCGA830.18050148968096905No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA810.17615205619468066No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGG800.17397733945153643No Hit
GATCGGAAGAGCACACGTCGAACTCCAGTCACCGTCGCCTATATCTAGTA780.16962790596524802No Hit
GGGGGAGAGAAAGAGAAAGAAGGGCGA770.16745318922210384No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA760.16527847247895963No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC740.1609290389926712No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC740.1609290389926712No Hit
GGGGGAAGGCCCGGTGGGGAAA700.15223017202009437No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACCGTCGCCTATATCTAGTA700.15223017202009437No Hit
GATCGGAAGAGCACACGTTGAACTCCAGTCACCGTCGCCTATATCTAGTA700.15223017202009437No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA690.15005545527695016No Hit
GGAGGAACGGGGGGCGCGAAGA680.14788073853380598No Hit
CGGGGAAGGCCCGGTGGGGAAA680.14788073853380598No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACCGTCGCCTATATCTAG670.14570602179066178TruSeq Adapter, Index 22 (95% over 24bp)
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACCGTCGCCTATATCTAG670.14570602179066178TruSeq Adapter, Index 22 (95% over 22bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC660.14353130504751757No Hit
GGCCCCCCCGGCAGCAGCACA660.14353130504751757No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA660.14353130504751757No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGA640.13918187156122913No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA630.13700715481808495No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC620.13483243807494075No Hit
GATCGGAAGAGCCACGTCTGAACTCCAGTCACCGTCGCCTATATCTAGTA610.13265772133179654TruSeq Adapter, Index 13 (95% over 24bp)
GATCGGAAGAGCACACGTCTGAATCCAGTCACCGTCGCCTATATCTAGTA600.1304830045886523Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA580.12613357110236392No Hit
GATCGGAAGAGCCACACGTCTGAACTCCAGTCACCGTCGCCTATATCTAG540.1174347041297871TruSeq Adapter, Index 22 (96% over 26bp)
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA540.1174347041297871No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACCGTCGCCTATATCTGG530.11525998738664289Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTAACTCCAGTCACCGTCGCCTATATCTAGTA530.11525998738664289Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CCAGCCCGACCGACCCAGCCCA510.11091055390035447No Hit
GCTGCTGCCGGGGGGGCCA500.10873583715721027No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCA490.10656112041406607Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGCCCCCCCGGCAGCAGCA490.10656112041406607No Hit
GCCCCCCCGGCAGCAGCA490.10656112041406607No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC490.10656112041406607No Hit
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACCGTCGCCTATATCTAG480.10438640367092186Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGACGCGGGGCCGGGGGGCA480.10438640367092186No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC480.10438640367092186No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG470.10221168692777766No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG470.10221168692777766No Hit
ATACGAATGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC460.10003697018463346No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGGG460.10003697018463346No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC460.10003697018463346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)