FastQCFastQC Report
Tue 3 Mar 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_3_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_3_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618078
Total Bases697.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC74

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTAGT232621150.37184300481715TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTGGT95126320.598677631690066TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTGGG1357452.9394263154498472TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTAGG1105512.393874681198542TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCGCTCGTATCTAGT837481.8134817125219627TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCGCTCGTATCTGGT684091.4813305448716978TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTAGTA418060.905268382214419TruSeq Adapter, Index 13 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTAGTATG281850.6103188382699469TruSeq Adapter, Index 13 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTGGTA180650.39118005369333303TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCGCTCGTATCTGGG129150.2796617986963408TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCGCTCGTATCTAGG110970.24029477198089766TruSeq Adapter, Index 13 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTGGTATG107320.23239105099567395TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACTCGTATCTAGT103100.22325305029494955TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACTCGTATCTGGT88080.1907286970900015TruSeq Adapter, Index 11 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTGGTG64970.14068623353698226TruSeq Adapter, Index 13 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCTAGTG57790.12513863992769286TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACNTCTGAACTCCAGTCACAGCCACTCGTATCTAGT54850.11877235507932087TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCANTCACAGCCACTCGTATCTAGT54340.11766799954439922TruSeq Adapter, Index 13 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCACTCGTATCGAGT53690.11626048758812649TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)