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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 6.12 250334217 4090325735
2 7.00 285165933 4074910534
3 6.21 253916096 4088175087
4 6.11 248089917 4059169203
5 6.55 268356261 4095544764
6 6.47 265289440 4099623814
7 6.78 278776905 4109400122
8 6.76 279032115 4129288043
2. SAMPLES WITH LOW READS (NON-NTC)
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No samples found with low reads
3. NTC SAMPLES WITH HIGH READS
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Found 2 NTC samples with high reads:
SampleID Index # Reads
1 NTC_0001_0001_B23LG7FLT4_2 CTCATCATCT-TAAGCCTCTA 1173175
0 NTC_0001_0001_B23LG7FLT4_1 CGTCGCCTAT-TATCTTCTCG 874575
4. TOP UNKNOWN BARCODES
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Found 126 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 AGCCTCTGCT GGGGGGGGGG 15462051 0.061766 0.003780 0.378015 0.047218
1 1 GCATTCCTTC GGGGGGGGGG 6799376 0.027161 0.001662 0.166231 0.020764
2 1 GCAGAAGTCT GGGGGGGGGG 6410016 0.025606 0.001567 0.156712 0.019575
3 1 GATAGATCAG GGGGGGGGGG 6303578 0.025181 0.001541 0.154109 0.019250
4 1 TCACCGTGGT GGGGGGGGGG 5979060 0.023884 0.001462 0.146176 0.018259
5 1 CGGATCATTA GGGGGGGGGG 4890689 0.019537 0.001196 0.119567 0.014935
6 1 TGTGATCGCG GGGGGGGGGG 4725038 0.018875 0.001155 0.115517 0.014429
7 1 GATTCTTAGC GGGGGGGGGG 4442279 0.017745 0.001086 0.108605 0.013566
8 1 AGCACCAGTC GGGGGGGGGG 3933604 0.015713 0.000962 0.096168 0.012012
9 1 GCGACAATAT GGGGGGGGGG 3606282 0.014406 0.000882 0.088166 0.011013
10 1 ATCGAGAGAC GGGGGGGGGG 3575489 0.014283 0.000874 0.087413 0.010919
11 1 CTAGACGAGC GGGGGGGGGG 3425352 0.013683 0.000837 0.083743 0.010460
12 1 CCACTCAACG GGGGGGGGGG 2883641 0.011519 0.000705 0.070499 0.008806
13 1 GCGAAGAATT GGGGGGGGGG 2872728 0.011476 0.000702 0.070232 0.008773
14 1 GTGTTCCTTC GAGCCTGCTT 2779782 0.011104 0.000680 0.067960 0.008489
15 1 ATCTAACCTG GGGGGGGGGG 2283440 0.009122 0.000558 0.055825 0.006973
16 1 CAACTACCGC GGGGGGGGGG 2195123 0.008769 0.000537 0.053666 0.006703
17 2 AGCCTCTGCT GGGGGGGGGG 13801734 0.048399 0.003387 0.338700 0.042147
18 2 GCATTCCTTC GGGGGGGGGG 6088094 0.021349 0.001494 0.149404 0.018592
19 2 GATAGATCAG GGGGGGGGGG 5722551 0.020067 0.001404 0.140434 0.017475
20 2 GCAGAAGTCT GGGGGGGGGG 5716443 0.020046 0.001403 0.140284 0.017457
21 2 TCACCGTGGT GGGGGGGGGG 5433855 0.019055 0.001333 0.133349 0.016594
22 2 CGGATCATTA GGGGGGGGGG 4351064 0.015258 0.001068 0.106777 0.013287
23 2 TGTGATCGCG GGGGGGGGGG 4190213 0.014694 0.001028 0.102830 0.012796
24 2 GATTCTTAGC GGGGGGGGGG 4160605 0.014590 0.001021 0.102103 0.012706
25 2 GCGACAATAT GGGGGGGGGG 3629263 0.012727 0.000891 0.089064 0.011083
26 2 AGCACCAGTC GGGGGGGGGG 3615647 0.012679 0.000887 0.088729 0.011041
27 2 ATCGAGAGAC GGGGGGGGGG 3338464 0.011707 0.000819 0.081927 0.010195
28 2 CTAGACGAGC GGGGGGGGGG 3281278 0.011507 0.000805 0.080524 0.010020
29 2 GCGAAGAATT GGGGGGGGGG 2703105 0.009479 0.000663 0.066335 0.008255
30 2 CCACTCAACG GGGGGGGGGG 2671012 0.009367 0.000655 0.065548 0.008157
31 2 GTGTTCCTTC GAGCCTGCTT 2654142 0.009307 0.000651 0.065134 0.008105
32 2 ATCTAACCTG GGGGGGGGGG 2143704 0.007517 0.000526 0.052607 0.006546
33 3 AGCCTCTGCT GGGGGGGGGG 13354033 0.052592 0.003267 0.326650 0.040780
34 3 GCATTCCTTC GGGGGGGGGG 5879199 0.023154 0.001438 0.143810 0.017954
35 3 GCAGAAGTCT GGGGGGGGGG 5775924 0.022747 0.001413 0.141284 0.017638
36 3 GATAGATCAG GGGGGGGGGG 5684881 0.022389 0.001391 0.139057 0.017360
37 3 TCACCGTGGT GGGGGGGGGG 5241177 0.020641 0.001282 0.128203 0.016005
38 3 CGGATCATTA GGGGGGGGGG 4394200 0.017306 0.001075 0.107486 0.013419
39 3 GATTCTTAGC GGGGGGGGGG 4241888 0.016706 0.001038 0.103760 0.012954
40 3 TGTGATCGCG GGGGGGGGGG 4086498 0.016094 0.001000 0.099959 0.012479
41 3 GCGACAATAT GGGGGGGGGG 3959008 0.015592 0.000968 0.096840 0.012090
42 3 AGCACCAGTC GGGGGGGGGG 3720865 0.014654 0.000910 0.091015 0.011363
43 3 CTAGACGAGC GGGGGGGGGG 3542724 0.013952 0.000867 0.086658 0.010819
44 3 ATCGAGAGAC GGGGGGGGGG 3431575 0.013515 0.000839 0.083939 0.010479
45 3 GCGAAGAATT GGGGGGGGGG 3037634 0.011963 0.000743 0.074303 0.009276
46 3 CCACTCAACG GGGGGGGGGG 2940399 0.011580 0.000719 0.071924 0.008979
47 3 GTGTTCCTTC GAGCCTGCTT 2835712 0.011168 0.000694 0.069364 0.008660
48 3 ATCTAACCTG GGGGGGGGGG 2392837 0.009424 0.000585 0.058531 0.007307
49 3 CGCACATTAC GGGGGGGGGG 2224352 0.008760 0.000544 0.054409 0.006793
50 3 CAACTACCGC GGGGGGGGGG 2171869 0.008553 0.000531 0.053126 0.006632
51 3 GATTGGCGAT GGGGGGGGGG 2155948 0.008491 0.000527 0.052736 0.006584
52 3 GTAATACTCG GGGGGGGGGG 2052798 0.008085 0.000502 0.050213 0.006269
53 4 AGCCTCTGCT GGGGGGGGGG 13111254 0.052849 0.003230 0.323003 0.040039
54 4 GCATTCCTTC GGGGGGGGGG 5785178 0.023319 0.001425 0.142521 0.017667
55 4 GCAGAAGTCT GGGGGGGGGG 5613130 0.022625 0.001383 0.138283 0.017141
56 4 GATAGATCAG GGGGGGGGGG 5466637 0.022035 0.001347 0.134674 0.016694
57 4 TCACCGTGGT GGGGGGGGGG 5065498 0.020418 0.001248 0.124791 0.015469
58 4 CGGATCATTA GGGGGGGGGG 4308240 0.017366 0.001061 0.106136 0.013156
59 4 TGTGATCGCG GGGGGGGGGG 4150257 0.016729 0.001022 0.102244 0.012674
60 4 GATTCTTAGC GGGGGGGGGG 3893699 0.015695 0.000959 0.095924 0.011890
61 4 AGCACCAGTC GGGGGGGGGG 3702627 0.014925 0.000912 0.091216 0.011307
62 4 GCGACAATAT GGGGGGGGGG 3452816 0.013918 0.000851 0.085062 0.010544
63 4 CTAGACGAGC GGGGGGGGGG 3401747 0.013712 0.000838 0.083804 0.010388
64 4 ATCGAGAGAC GGGGGGGGGG 3169982 0.012778 0.000781 0.078094 0.009680
65 4 GCGAAGAATT GGGGGGGGGG 3151401 0.012703 0.000776 0.077637 0.009624
66 4 CCACTCAACG GGGGGGGGGG 2927500 0.011800 0.000721 0.072121 0.008940
67 4 GTGTTCCTTC GAGCCTGCTT 2871127 0.011573 0.000707 0.070732 0.008768
68 4 ATCTAACCTG GGGGGGGGGG 2388460 0.009627 0.000588 0.058841 0.007294
69 4 GATTGGCGAT GGGGGGGGGG 2243153 0.009042 0.000553 0.055261 0.006850
70 4 CAACTACCGC GGGGGGGGGG 2233873 0.009004 0.000550 0.055033 0.006822
71 4 CGCACATTAC GGGGGGGGGG 2145749 0.008649 0.000529 0.052862 0.006553
72 5 GTCGACTCCT GGGGGGGGGG 28213535 0.105135 0.006889 0.688884 0.086158
73 5 CCTCTGTCAT GGGGGGGGGG 21211324 0.079042 0.005179 0.517912 0.064774
74 5 TTGTGAAGGC GGGGGGGGGG 17124222 0.063812 0.004181 0.418118 0.052293
75 5 CTTCGGAATC GGGGGGGGGG 9047527 0.033715 0.002209 0.220911 0.027629
76 5 GCAGATGTAA GGGGGGGGGG 6508182 0.024252 0.001589 0.158909 0.019874
77 5 CAACCATACA GGGGGGGGGG 6450505 0.024037 0.001575 0.157501 0.019698
78 5 TTGCAATGCG GGGGGGGGGG 5469904 0.020383 0.001336 0.133557 0.016704
79 5 CATTATCGCT GGGGGGGGGG 5383290 0.020060 0.001314 0.131443 0.016439
80 5 AACATAAGGC GGGGGGGGGG 3805274 0.014180 0.000929 0.092913 0.011620
81 5 CGGATTGATC GGGGGGGGGG 3792311 0.014132 0.000926 0.092596 0.011581
82 5 ACTTAGATCG GGGGGGGGGG 2963370 0.011043 0.000724 0.072356 0.009049
83 5 AACCGGCCTT GGGGGGGGGG 2671844 0.009956 0.000652 0.065238 0.008159
84 5 AGGTATTCGG AGATCTCGGT 2576643 0.009602 0.000629 0.062913 0.007868
85 5 AAGAGTACGA GGGGGGGGGG 2185456 0.008144 0.000534 0.053362 0.006674
86 6 GTCGACTCCT GGGGGGGGGG 31161329 0.117462 0.007601 0.760102 0.095159
87 6 CCTCTGTCAT GGGGGGGGGG 20161615 0.075999 0.004918 0.491792 0.061569
88 6 TTGTGAAGGC GGGGGGGGGG 18902057 0.071251 0.004611 0.461068 0.057722
89 6 CTTCGGAATC GGGGGGGGGG 9912851 0.037366 0.002418 0.241799 0.030272
90 6 CAACCATACA GGGGGGGGGG 5813006 0.021912 0.001418 0.141794 0.017752
91 6 TTGCAATGCG GGGGGGGGGG 5404782 0.020373 0.001318 0.131836 0.016505
92 6 CATTATCGCT GGGGGGGGGG 5307486 0.020006 0.001295 0.129463 0.016208
93 6 GCAGATGTAA GGGGGGGGGG 5050759 0.019039 0.001232 0.123201 0.015424
94 6 AACATAAGGC GGGGGGGGGG 3620816 0.013649 0.000883 0.088321 0.011057
95 6 CGGATTGATC GGGGGGGGGG 3458507 0.013037 0.000844 0.084362 0.010561
96 6 ACTTAGATCG GGGGGGGGGG 3057781 0.011526 0.000746 0.074587 0.009338
97 6 AACCGGCCTT GGGGGGGGGG 2391704 0.009015 0.000583 0.058340 0.007304
98 6 AGGTATTCGG AGATCTCGGT 2384264 0.008987 0.000582 0.058158 0.007281
99 7 GTCGACTCCT GGGGGGGGGG 33418063 0.119874 0.008132 0.813210 0.102051
100 7 CCTCTGTCAT GGGGGGGGGG 22375267 0.080262 0.005445 0.544490 0.068329
101 7 TTGTGAAGGC GGGGGGGGGG 20975773 0.075242 0.005104 0.510434 0.064055
102 7 CTTCGGAATC GGGGGGGGGG 10655032 0.038221 0.002593 0.259284 0.032538
103 7 CAACCATACA GGGGGGGGGG 6528282 0.023418 0.001589 0.158862 0.019936
104 7 TTGCAATGCG GGGGGGGGGG 6130695 0.021991 0.001492 0.149187 0.018722
105 7 GCAGATGTAA GGGGGGGGGG 5931573 0.021277 0.001443 0.144342 0.018114
106 7 CATTATCGCT GGGGGGGGGG 5737349 0.020580 0.001396 0.139615 0.017521
107 7 AACATAAGGC GGGGGGGGGG 4069480 0.014598 0.000990 0.099029 0.012427
108 7 CGGATTGATC GGGGGGGGGG 3947189 0.014159 0.000961 0.096053 0.012054
109 7 ACTTAGATCG GGGGGGGGGG 3385918 0.012146 0.000824 0.082394 0.010340
110 7 AACCGGCCTT GGGGGGGGGG 2644753 0.009487 0.000644 0.064359 0.008076
111 7 AGGTATTCGG AGATCTCGGT 2070736 0.007428 0.000504 0.050390 0.006324
112 7 AAGAGTACGA GGGGGGGGGG 2058586 0.007384 0.000501 0.050095 0.006286
113 8 GTCGACTCCT GGGGGGGGGG 34256889 0.122770 0.008296 0.829608 0.104613
114 8 CCTCTGTCAT GGGGGGGGGG 22855710 0.081911 0.005535 0.553502 0.069796
115 8 TTGTGAAGGC GGGGGGGGGG 21380948 0.076625 0.005178 0.517788 0.065292
116 8 CTTCGGAATC GGGGGGGGGG 11044249 0.039581 0.002675 0.267461 0.033727
117 8 TTGCAATGCG GGGGGGGGGG 6330099 0.022686 0.001533 0.153298 0.019331
118 8 CAACCATACA GGGGGGGGGG 6312569 0.022623 0.001529 0.152873 0.019277
119 8 GCAGATGTAA GGGGGGGGGG 5877037 0.021062 0.001423 0.142326 0.017947
120 8 CATTATCGCT GGGGGGGGGG 5828140 0.020887 0.001411 0.141142 0.017798
121 8 AACATAAGGC GGGGGGGGGG 4069191 0.014583 0.000985 0.098545 0.012426
122 8 CGGATTGATC GGGGGGGGGG 3901058 0.013981 0.000945 0.094473 0.011913
123 8 ACTTAGATCG GGGGGGGGGG 3521385 0.012620 0.000853 0.085278 0.010753
124 8 AACCGGCCTT GGGGGGGGGG 2574437 0.009226 0.000623 0.062346 0.007862
125 8 AAGAGTACGA GGGGGGGGGG 2103096 0.007537 0.000509 0.050931 0.006422
5. BARCODES WITH HIGH MISMATCH RATES
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Found 4 barcodes with high mismatch rates:
Lane SampleID Index # Reads Mismatch_Rate
178 2 FFPE_V4_0001_RNA_0001_B23LG7FLT4_1 GCATTCCTTC-GAGCCTGCTT 69597426 27.967872
31 3 FFPE_V4_0001_RNA_0001_B23LG7FLT4_1 GCATTCCTTC-GAGCCTGCTT 70368438 26.902550
80 4 FFPE_V4_0001_RNA_0001_B23LG7FLT4_1 GCATTCCTTC-GAGCCTGCTT 69620090 26.320714
129 1 FFPE_V4_0001_RNA_0001_B23LG7FLT4_1 GCATTCCTTC-GAGCCTGCTT 70409954 25.772042
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FINAL STATUS CHECK
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Overall % Undetermined: 6.50%
Status: FAIL
Reason(s):
Overall undetermined 6.50% >= 5.0%
NTC sample NTC_0001_0001_B23LG7FLT4_1 has 874,575 reads > 500,000
NTC sample NTC_0001_0001_B23LG7FLT4_2 has 1,173,175 reads > 500,000