FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3995385
Total Bases603.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC75

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGG255998364.0734998003947TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGT55858313.980705238669113TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTAGG973352.4361857493082644TruSeq Adapter, Index 8 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG483461.2100460906771189TruSeq Adapter, Index 8 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTAGT398150.9965247404192588TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCGGGG145770.36484594100443385TruSeq Adapter, Index 8 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGGTG145060.36306889073268284TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGA110870.2774951600408972TruSeq Adapter, Index 8 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGGGG102950.25767228940389975TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATTTGGG82170.2056622828588484TruSeq Adapter, Index 8 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATGTGGG70060.17535231273081317TruSeq Adapter, Index 8 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGTG52350.131026171445305TruSeq Adapter, Index 8 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA49530.12396802811243472No Hit
GATCGGAAGAGCACACGTCTGAACTCCANTCACACTCGTCCAGATCTGGG48980.12259143987375434TruSeq Adapter, Index 8 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGATG47720.11943780136332294TruSeq Adapter, Index 8 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG42890.10734885374000254TruSeq Adapter, Index 8 (96% over 31bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC42300.10587214999305447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)