FastQCFastQC Report
Thu 28 May 2026
OvarianN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOvarianN_FFPE_L03_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6491904
Total Bases980.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTAGT218361933.63603343487519TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGT165563425.5030573465042TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGG3987606.142419850940495TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTAGG2564083.9496579123782483TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGTG171580.26429842462242203TruSeq Adapter, Index 7 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTAGTA146070.22500332722110494TruSeq Adapter, Index 7 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTAGTG122020.18795718482590007TruSeq Adapter, Index 7 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTAGTATG99240.1528673252099846TruSeq Adapter, Index 7 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGTA96520.14867749122599472TruSeq Adapter, Index 7 (97% over 34bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGTGTG83850.12916087483733585TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCGGGT78530.12096605248629677TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCGAGT76090.11720752494183524TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATGTGGT67150.10343652647975077TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTAGACTTATCTGGGG65260.10052520801293427TruSeq Adapter, Index 7 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)