FastQCFastQC Report
Thu 28 May 2026
22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17658335
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCATGATATCTAGT235702313.347934558949074TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCATGATATCTGGT7047163.9908405860461933TruSeq Adapter, Index 23 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCATGATATCTAGTA427180.24191408759659389TruSeq Adapter, Index 23 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAACCATGATATCTAGTATG248500.14072674462229876TruSeq Adapter, Index 23 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCATGATATCTGGG239580.13567530574088668TruSeq Adapter, Index 23 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)