FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_2_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_2_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149272
Total Bases9.9 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA29942.0057344980974325No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA27641.8516533576290264TruSeq Adapter, Index 1 (96% over 29bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC20641.3827107562034406No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT19141.282223055897958No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG12340.8266788145131037No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA11110.7442789002626078No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGTA10270.6880057880915376TruSeq Adapter, Index 1 (96% over 29bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC8610.5767993997534702No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC5600.37515408114046844No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACAAGGCTCCATATCTAGTA5210.3490272790610429Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACGGGAAGGCCCGGTGGGGAAA4810.3222305589795809No Hit
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA4460.2987834289083016No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4310.28873465887775335No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAAGGCTCCATATCTAGTA4120.2760062168390589Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAG3640.24385015274130448TruSeq Adapter, Index 1 (96% over 30bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA3540.23715097272093894No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC3450.23112171070261No Hit
GCCCCCCCGGCAGCAGCACA3300.22107294067206176No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA3100.20767458063133074No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGGA3080.20633474462725762No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGGTG2910.19494613859263626TruSeq Adapter, Index 1 (96% over 29bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2820.1889168765743073No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAAGGCTCCATATCTAG2740.1835575325580149Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2660.1781981885417225No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2640.1768583525376494No Hit
GCTGCTGCCGGGGGGCCCA2560.171499008521357No Hit
GGCCCCCCCGGCAGCAGCA2560.171499008521357No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA2560.171499008521357No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTG2510.16814941851117424TruSeq Adapter, Index 1 (96% over 29bp)
GGAGGAACGGGGGGCGCGAAGA2490.16680958250710115No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACAAGGCTCCATATCTAGTA2480.1661396645050646Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CACGGGAAGGCCCGGTGGGGAAA2460.16479982850099148No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA2450.16412991049895492No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2420.16212015649284528No Hit
GATCGGAAGAGCACCGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA2380.15944048448469908TruSeq Adapter, Index 7 (95% over 21bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACAAGGCTCCATATCTAGTA2290.1534112224663701No Hit
GCTGCTGCCGGGGGGGCCA2270.152071386462297No Hit
GCCCCCCCGGCAGCAGCA2250.15073155045822392No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCA2240.15006163245618737No Hit
GGCCCCCCCGGCAGCAGCACA2210.14805187845007772No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA2150.1440323704378584TruSeq Adapter, Index 1 (95% over 21bp)
GCCCCGGGGGCGGACCCGAAAGATGGA2130.1426925344337853No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC2120.14202261643174874No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACAAGGCTCCATATCTAGTA2090.1400128624256391No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACAAGGCTCCATATCTAGTA1990.13331368240527358Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CGGGGAAGGCCCGGTGGGGAAA1990.13331368240527358No Hit
GCTGCTGCCGGGGGGGCA1970.1319738464012005No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC1930.1292941743930543No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGA1870.12527466638083498No Hit
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAAGGCTCCATATCTGG1860.12460474837879844TruSeq Adapter, Index 1 (96% over 30bp)
GGGGGAGAGAGAGAGAGAGAGGGCGCGA1840.12326491237472532No Hit
GGGGGAGAGAAAGAGAAAGAAGGGCGA1830.1225949943726888No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACAAGGCTCCATATCTAGTA1820.12192507637065224No Hit
GGGAGAGAGAGAGAGAGAGGGCGCGAAGA1770.11857548636046947No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAAGGCTCCATATCTGGTA1760.11790556835843294Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTAACTCCAGTCACAAGGCTCCATATCTAGTA1740.11656573235435982Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACAAGGCTCCATATCTGGTA1730.11589581435232327Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACCCAGTCACAAGGCTCCATATCTAGTA1680.11254622434214052Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGGGGAGAGAGAGAGAGAGAGGGCGCGAAG1680.11254622434214052No Hit
GGACGCGGGGCCGGGGGGCCCA1680.11254622434214052No Hit
GATCGGAAGAGCACACGTTGAACTCCAGTCACAAGGCTCCATATCTAGTA1660.11120638833806741No Hit
GATCGGAAGAGCACACGTCTGAATCCAGTCACAAGGCTCCATATCTAGTA1650.11053647033603088Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA1590.10651696232381155No Hit
GGGGGAAGGCCCGGTGGGGAAA1550.10383729031566537No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCA1520.1018275363095557Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC1510.10115761830751917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)