FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_2_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_2_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149272
Total Bases10.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720311.524599389034783No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC33892.27035210890187No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA31742.126319738464012No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC10890.7295407042178037No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG8710.5834985797738357No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC8600.5761294817514336No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5480.3671150651160298No Hit
TTCCCCACCGGGCCTTCCCGT4780.3202208049734713No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC4400.29476392089608233No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC4340.29074441288386305No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC3580.23983064472908516No Hit
GTGCTGCTGCCGGGGGGGC3320.22241277667613482No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC3290.2204030226700252No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG3180.2130339246476231No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG2790.18690712256819764No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC2790.18690712256819764No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC2720.1822176965539418No Hit
GCTGCTGCCGGGGGGGCC2530.16948925451524735No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC2530.16948925451524735No Hit
GGGCCCCCCGGCAGCAGC2510.16814941851117424No Hit
CTTCGCGCCCCCCGTTCCTCC2460.16479982850099148No Hit
TTCCCCACCGGGCCTTCCCGTG2420.16212015649284528No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC2380.15944048448469908No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC2350.15743073047858944No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC2250.15073155045822392No Hit
GCTGCTGCCGGGGGGGC2240.15006163245618737No Hit
GGCCCCCCCGGCAGCAGC2230.1493917144541508No Hit
GTGCTGCTGCCGGGGGGGCC2160.14470228843989497No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG2130.1426925344337853No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC2090.1400128624256391No Hit
TTCCCCACCGGGCCTTCCCCG2000.13398360040731014No Hit
GCCCCCCCGGCAGCAGC1980.13264376440323702No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC1970.1319738464012005No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG1880.12594458438287154No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCC1820.12192507637065224No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCC1790.11991532236454258No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCC1730.11589581435232327No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC1720.11522589635028672No Hit
GCCCCCCGGCCCCGCGTCC1650.11053647033603088No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCCG1630.10919663433195777No Hit
GGGCCCCCCGGCCCCGCGTCC1620.10852671632992122No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG1560.10450720831770191No Hit
CCGCCCCCCGGCCCCGCGTCC1520.1018275363095557No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG1510.10115761830751917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)