FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315474
Total Bases18.2 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302179.578285373755048No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA65342.0711690979288306No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC53001.680011664986655No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC26530.8409567824923766No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC23660.7499825659166841No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC17630.558841616107825No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG16260.5154148994845852No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC14400.456455999543544No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC13510.4282444829050889No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC10020.3176172996823827No Hit
TTCCCCACCGGGCCTTCCCGT9770.30969271635697393No Hit
GTGCTGCTGCCGGGGGGGC9260.29352656637314006No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC8250.2615112497384887No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC7360.2332997331000336No Hit
CTTCGCGCCCCCCGTTCCTCC7090.22474118310859215No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC6960.22062039977937958No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC6920.2193524664473142No Hit
GTGCTGCTGCCGGGGGGGCC6320.2003334664663332No Hit
TTCCCCACCGGGCCTTCCCGTG6310.20001648313331685No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6000.19018999980981No Hit
GGCCCCCCCGGCAGCAGC5870.18606921648059746No Hit
CCGCCCCCCGGCCCCGCGTCC5530.17529178315804156No Hit
GCTGCTGCCGGGGGGGCC5480.1737068664929598No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG5430.17212194982787804No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC5410.17148798316184535No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG5320.1686351331646982No Hit
GGGCCCCCCGGCAGCAGC5300.1680011664986655No Hit
GGGCCCCCCGGCCCCGCGTCC5030.15944261650722405No Hit
GCCCCCCGGCCCCGCGTCC5020.1591256331742077No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG4860.1540538998459461No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC4780.15151803318181528No Hit
GCTGCTGCCGGGGGGGC4720.14961613318371722No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCC4620.1464462998535537No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC4590.14549534985450466No Hit
GGACGCGGGGCCGGGGGGCGG4580.1451783665214883No Hit
GCCCCCCCGGCAGCAGC4510.14295948319037385No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC4340.1375707665290959No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCC4300.13630283319703052No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG4270.13535188319798144No Hit
CCATCTTTCGGGTCCGCCCCCG4160.1318650665348016No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG3880.1229895332103438No Hit
CCCGCCCCCCGGCCCCGCGTCC3840.1217215998782784No Hit
GGACGCGGGGCCGGGGGGC3740.1185517665481149No Hit
CCATCTTTCGGGTCCGCCCCCGG3620.1147479665519187No Hit
CCATCTTTCGGGTCCGCCCCCGGG3560.1128460665538206No Hit
CTTCGCGCCCTCTCTCTCTCTCTCCCCC3480.11031019988968979No Hit
ACCGTGCCCTCTCTCTCTCTCTCTCCCCC3420.1084082998915917No Hit
TTCCCCACCGGGCCTTCCCCG3410.10809131655857535No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC3400.10777433322555899No Hit
GGCCCCCCGGCCCCGCGTCC3350.10618941656047726No Hit
GCCCCGGGGGCGGACCCGAAAG3330.10555544989444454No Hit
CGCCCTTCTTTCTCTTTCTCTCCCCC3320.1052384665614282No Hit
CTTTCATCTTTCCCGCCCCCCGTTCCTCC3200.10143466656523199No Hit
CTTCGCCCCCCGGCCCCGCGTC3190.10111768323221565No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)