FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315474
Total Bases17.3 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC66

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA48371.533248381800085No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC42141.3357677653308988No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG41441.3135789320197544TruSeq Adapter, Index 8 (96% over 31bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT36781.1658646988341352No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA26870.8517342158149324No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC23840.7556882659109784No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG19010.6025853160640814No Hit
GCCCCGGGGGCGGACCCGAAAGATGGA17820.5648642994351357No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC14980.4748410328584923No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACACTCGTCCAGATCTGGGG13730.43521811623144857Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC13520.4285614662381052No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACACTCGTCCAGATCTGGGG11440.3626289329707044Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC10270.3255418830077914No Hit
ACGGGAAGGCCCGGTGGGGAAA9830.311594616355072No Hit
GCCCCCCCGGCAGCAGCACA9280.29416053303917283No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACACTCGTCCAGATCTGGGG9030.28623594971376404Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGGAGAGAGAGAGAGAGAGGGCGCGAAGA8490.2691188497308811No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGA7620.2415412997584587TruSeq Adapter, Index 8 (96% over 31bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACACTCGTCCAGATCTGG7320.2320317997679682TruSeq Adapter, Index 8 (96% over 32bp)
GGGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC7240.2294959331038374No Hit
GGAGGAACGGGGGGCGCGAAGA7190.22791101643875566No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC6950.22030341644636323No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGA6900.2187184997812815No Hit
GGCCCCCCCGGCAGCAGCACA6540.20730709979269288No Hit
CACGGGAAGGCCCGGTGGGGAAA6470.20508821646157846No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCA6210.19684664980315333No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC6150.19494474980505527No Hit
GCTGCTGCCGGGGGGGCCA5920.1876541331456792No Hit
GATCGGAAGAGCACAGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG5730.18163144981836857TruSeq Adapter, Index 8 (95% over 21bp)
GGCCCCCCCGGCAGCAGCA5550.17592574982407425No Hit
GGACGCGGGGCCGGGGGGCGGA5490.17402384982597613No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACACTCGTCCAGATCTGGGG5390.17085401649581264Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5370.17022004982977995No Hit
GCTGCTGCCGGGGGGCCCA5360.16990306649676362No Hit
GATCGGAAGAGCAACGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG5170.16388038316945294TruSeq Adapter, Index 8 (95% over 23bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGA5110.16197848317135485No Hit
GGACGCGGGGCCGGGGGGCCCA5030.15944261650722405No Hit
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACACTCGTCCAGATCTGG5020.1591256331742077Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGCTGAACTCCAGTCACACTCGTCCAGATCTGGGG5020.1591256331742077No Hit
GATCGGAAGAGCACCGTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG4980.1578576998421423TruSeq Adapter, Index 8 (95% over 23bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACACTCGTCCAGATCTGGGG4970.15754071650912596No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4900.1553218331780115No Hit
GAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC4830.15310294984689704No Hit
GCCCCCCCGGCAGCAGCA4760.1508840665157826No Hit
CTCGCGGGGGATTCCCCGCGAGG4760.1508840665157826No Hit
CCGCCCCCCGGCCCCGCGTCCA4670.14803121651863546No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGA4650.14739724985260275No Hit
CGGGGGCGGACCCGAAAGATGGA4440.1407405998592594No Hit
GCTGCTGCCGGGGGGGCA4440.1407405998592594No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCA4420.1401066331932267No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA4380.1388386998611613No Hit
GGGAGAGAGAGAGAGAGAGGGCGCGAAGA4360.1382047331951286No Hit
GATCGGAAGAGCACACGTCTAACTCCAGTCACACTCGTCCAGATCTGGGG4330.13725378319607956Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA4180.1324990332008343No Hit
GGACGCGGGGCCGGGGGGCA4080.12932919987067082No Hit
GATCGGAAGAGCACACGTCTGAATCCAGTCACACTCGTCCAGATCTGGGG4010.12711031653955635Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC3970.12584238320749094No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACACTCGTCCAGATCTGGGG3950.12520841654145826TruSeq Adapter, Index 8 (95% over 21bp)
GCCCCCCGGCCCCGCGTCCA3800.120453666546213No Hit
GATCGGAAGAGCACACGTCTGAACCCAGTCACACTCGTCCAGATCTGGGG3760.11918573321414759Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGACGCGGGGCCGGGGGGCGGGA3690.11696684988303314No Hit
CCCGGGGGCGGACCCGAAAGATGGA3620.1147479665519187No Hit
CCGGGGGCGGACCCGAAAGATGGA3580.11348003321985331No Hit
GGGGGAGAGAGAGAGAGAGAGGGCACGGTA3570.11316304988683695No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACACTCGTCCAGATCTGGGA3550.11252908322080424Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGAGAGAGAGAGAGAGGGCGCGAAGA3470.10999321655667345No Hit
GATCGGAAGAGCACACGTTGAACTCCAGTCACACTCGTCCAGATCTGGGG3470.10999321655667345No Hit
CGGGGAAGGCCCGGTGGGGAAA3420.1084082998915917No Hit
GGGGGAGAGAAAGAGAAAGAAGGGCGA3370.10682338322650996No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC3340.10587243322746089No Hit
CTTTCGGGTCCGCCCCCGGGGCA3340.10587243322746089No Hit
GGACGCGGGGCCGGGGGGCCA3320.1052384665614282No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGCCC3280.10397053322936281No Hit
GGAGGAACGGGGGGCGGGAAAGATGAAAGA3200.10143466656523199No Hit
GACGCGGGGCCGGGGGGCGAAGA3180.10080069989919929No Hit
CGGGGGAGAGAGAGAGAGAGAGGGCGCGAAG3170.10048371656618295No Hit
GGGGGAAGGCCCGGTGGGGAAA3160.1001667332331666No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)