FastQCFastQC Report
Thu 28 May 2026
A549_FFPE_01_RNA_0001_B23LG7FLT4_3_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameA549_FFPE_01_RNA_0001_B23LG7FLT4_3_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393174
Total Bases21.1 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC77

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4001210.176664784548317No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC48081.222868246628719No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA41731.0613621450045019No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC30620.7787900522415012No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC24220.6160122490296918No Hit
CCATCTTTCGGGTCCGCCCCCGGGGC21530.547594703617228No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC21030.5348776877413054No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC15990.4066901677120054No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC15300.38914068580323213No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAGC14840.37744103119738337No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGCAG11050.2810460508578899No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG10840.2757049041900024No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCC10780.2741788622848917No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGG10390.26425958990167203No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC9900.25179691434326784No Hit
GTGCTGCTGCCGGGGGGGC9340.23755385656223454No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGG8760.22280211814616427No Hit
CCGCCCCCCGGCCCCGCGTCC8730.22203909719360893No Hit
TTCCCCACCGGGCCTTCCCGT8220.2090677410001679No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCC8090.205761316872428No Hit
GGAACCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC8080.20550697655490952No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC7980.202963573379725No Hit
GGGCCCCCCGGCCCCGCGTCC7800.19838544766439284No Hit
GCCCCCCGGCCCCGCGTCC7690.1955877041716899No Hit
CTGCTGCCGGGGGGGCTGTAACACTCGGG7320.18617711242350715No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTCCC7140.181598986708175No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAGC7010.17829256258043513No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG6830.173714436865103No Hit
GGGCCCCCCGGCAGCAGC6520.16582988702203094No Hit
GCTGCTGCCGGGGGGGC6460.16430384511692023No Hit
GTGCTGCTGCCGGGGGGGCC6410.163032143529328No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC6400.16277780321180954No Hit
CTTCGCGCCCCCCGTTCCTCC6160.15667363559136668No Hit
GCCCCCCCGGCAGCAGC6140.15616495495632976No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC6070.1543845727337006No Hit
CCCGCCCCCCGGCCCCGCGTCC6060.15413023241618215No Hit
GGCCCCCCCGGCAGCAGC6000.15260419051107144No Hit
GCTGCTGCCGGGGGGGCC5890.14980644701836845No Hit
CCATCTTTCGGGTCCGCCCCCG5750.14624568257311013No Hit
GGGCCCCCCGAGTGTTACAGCCCCCCCGGCAGC5540.14090453590522262No Hit
CCCACCCCCGCGGGGAATCCCCC5530.14065019558770417No Hit
CCCCGTCCTCCCCCCTCCCCGGGG5530.14065019558770417No Hit
GGGGGATTCCCCGCGGGGGTGGG5510.14014151495266727No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGTTC5460.138869813365075No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5380.1368350908249274No Hit
CCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5360.13632641018989047No Hit
CCCCCGGCCCCGCGTCC5360.13632641018989047No Hit
CCGAGTGTTACAGCCCCCCCGGCAGCAG5260.13378300701470594No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC5250.1335286666971875No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAG5090.12945922161689227No Hit
CCATCTTTCGGGTCCGCCCCCGG5030.12793317971178153No Hit
GGACGCGGGGCCGGGGGGCGG4950.12589845717163392No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG4890.1243724152665232No Hit
GGACGCGGGGCCGGGGGGC4810.1223376927263756No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCC4790.12182901209133869No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCC4730.12030297018622799No Hit
CCATCTTTCGGGTCCGCCCCCGGG4700.11953994923367263No Hit
TTCCCCACCGGGCCTTCCCGTG4590.11674220574096966No Hit
GCCCCGGGGGCGGACCCGAAAG4550.11572484447089583No Hit
GCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4540.11547050415337738No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG4530.11521616383585893No Hit
CCCGAGTGTTACAGCCCCCCCGGCAGC4480.11394446224826667No Hit
GGGGGATTCCCCGCGGGGGTGG4460.11343578161322977No Hit
CCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4410.11216408002563749No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGC4280.10885765589789761No Hit
GGCCCCCCGGCCCCGCGTCC4280.10885765589789761No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCCG4240.10784029462782381No Hit
CCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC4210.10707727367526845No Hit
ACCGTGCCCTCTCTCTCTCTCTCTCCCCC4110.10453387050008395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGG4100.10427953018256547No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.10377084954752858No Hit
CTTTCGGGTCCGCCCCCGGGGC4010.10199046732489941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)