FastQCFastQC Report
Thu 28 May 2026
BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBreastNP_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15162667
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTAGT359234623.69204573311542TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTGGT11932037.869347786903188TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTAGTA587080.3871878212454313TruSeq Adapter, Index 19 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTAGTATG373520.24634188695168202TruSeq Adapter, Index 19 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTGGG245680.1620295426919288TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAACGTATATCTGGTA232010.1530139783456301TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)