FastQCFastQC Report
Thu 28 May 2026
EndoN_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameEndoN_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17448653
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTGGG199227311.417918620996131TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTAGG5828723.3404985473663786TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTGGT3573322.047905932910695TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTAGT1652810.9472421739374381TruSeq Adapter, Index 22 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTGGGG466180.2671724860366012TruSeq Adapter, Index 22 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCAATAAGAATCTGGGGGG236460.13551762419712285TruSeq Adapter, Index 22 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)