FastQCFastQC Report
Thu 28 May 2026
OvarianNP_FFPE_L01_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOvarianNP_FFPE_L01_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16695125
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC62

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTGGG379336222.721375251757625TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTAGG8466455.071210907375656TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTGGT5078863.042121577406578TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTAGT2139661.2816076549292084TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTGGGG733350.43925996361213226TruSeq Adapter, Index 18 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGTACAGCGGAATCTGGGGGG271550.162652271246846TruSeq Adapter, Index 18 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)