FastQCFastQC Report
Thu 28 May 2026
OvarianNP_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameOvarianNP_FFPE_L02_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17839955
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCAACAGATCTGGT286737016.0727423359532TruSeq Adapter, Index 8 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCAACAGATCTGGG202211111.334731505768932TruSeq Adapter, Index 8 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCAACAGATCTAGT1634030.9159384090374667TruSeq Adapter, Index 8 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCAACAGATCTAGG1030440.5776023538175965TruSeq Adapter, Index 8 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTCAACAGATCTGGGG375280.21035927500938204TruSeq Adapter, Index 8 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)