FastQCFastQC Report
Thu 28 May 2026
HeadNeckN_FFPE_L05_RNA_01_B23LG7FLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHeadNeckN_FFPE_L05_RNA_01_B23LG7FLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23355997
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGT547414723.437864801917897TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGT6469262.769849645039773TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGG1074430.46002317948576554TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTGGG790860.3386111070317401TruSeq Adapter, Index 6 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTG497650.21307161496895208TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACAGGTCATCTAGTA251960.10787807516844602TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)