STAR-FUSION FILTERING SUMMARY ================================================================================ Input file: SkinN_FFPE_L09_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.tsv Blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz Filter config: starfusion_filter_config.yaml Output file: SkinN_FFPE_L09_RNA_01_B23LG7FLT4_1.starfusion.starfusion.fusion_predictions.filter_summary.tsv Total fusions: 1581 Pass (CALL=1): 1 (0.1%) Fail (CALL=0): 1580 (99.9%) Blacklist Flagging: Total blacklisted: 152 All blacklisted fusions have CALL=0 Failure Reasons: Excluded SpliceType: INCL_NON_REF_SPLICE: 770 Read count too low: 1 < 8: 567 Blacklisted: 70 Read count too low: 2 < 8: 48 Blacklisted; aih_curated_blacklist score(3): 41 aih_curated_blacklist score(3): 37 Excluded annotation pattern: HGNC_GENEFAM: 30 Excluded annotation pattern: GTEx_recurrent_StarF2019: 4 aih_curated_blacklist score(5): 4 Excluded annotation pattern: DGD_PARALOGS: 3 Read count too low: 3 < 8: 3 Read count too low: 7 < 8: 2 Read count too low: 4 < 8: 1 Breakdown by SpliceType: INCL_NON_REF_SPLICE: 0 pass, 942 fail (total: 942) ONLY_REF_SPLICE: 1 pass, 638 fail (total: 639)