+ mkdir BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1 + all_qc -bam BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai 92058it [00:00, 920497.48it/s] 191681it [00:00, 964997.55it/s] 292279it [00:00, 983677.36it/s] 390647it [00:00, 896708.52it/s] 481356it [00:00, 822668.06it/s] 564956it [00:00, 787611.35it/s] 644597it [00:00, 770876.79it/s] 722197it [00:00, 757149.00it/s] 798202it [00:00, 749818.20it/s] 873346it [00:01, 743009.51it/s] 947733it [00:01, 738391.18it/s] 1021616it [00:01, 735239.31it/s][W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai 1095161it [00:01, 729905.29it/s] 1169634it [00:01, 734193.89it/s] 1243993it [00:01, 736515.34it/s] 1317661it [00:01, 730786.38it/s] 1390754it [00:01, 723112.27it/s][W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai 1464535it [00:01, 727436.12it/s] 1537804it [00:02, 728978.42it/s] 1611215it [00:02, 730440.37it/s] 1684272it [00:02, 729628.80it/s][W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai 1762491it [00:02, 745307.94it/s] 1855542it [00:02, 800665.69it/s] 1950857it [00:02, 846281.79it/s] 2071338it [00:02, 953513.40it/s] 2168269it [00:02, 958230.11it/s] 2279216it [00:02, 1003527.07it/s] 2379593it [00:02, 984447.87it/s] 2432244it [00:02, 820608.88it/s] [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md.bai + merge_qc -folders BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1/BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1.md -output BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1/BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1_full_basic_stats.tsv + head -n1 BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1/BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1/BreastNB_FFPE_L02_RNA_01_B23LG7FLT4_1_full_basic_stats.tsv