[W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15729307) splicing reads. 249002 splicing junctions. 170059 known splicing junctions. 78943 novel splicing junctions.
sampling 10% (31458615) splicing reads. 310454 splicing junctions. 182695 known splicing junctions. 127759 novel splicing junctions.
sampling 15% (47187923) splicing reads. 355552 splicing junctions. 189924 known splicing junctions. 165628 novel splicing junctions.
sampling 20% (62917231) splicing reads. 391953 splicing junctions. 194703 known splicing junctions. 197250 novel splicing junctions.
sampling 25% (78646539) splicing reads. 422322 splicing junctions. 198318 known splicing junctions. 224004 novel splicing junctions.
sampling 30% (94375847) splicing reads. 448119 splicing junctions. 201010 known splicing junctions. 247109 novel splicing junctions.
sampling 35% (110105154) splicing reads. 470855 splicing junctions. 203242 known splicing junctions. 267613 novel splicing junctions.
sampling 40% (125834462) splicing reads. 490689 splicing junctions. 205103 known splicing junctions. 285586 novel splicing junctions.
sampling 45% (141563770) splicing reads. 508172 splicing junctions. 206641 known splicing junctions. 301531 novel splicing junctions.
sampling 50% (157293078) splicing reads. 523894 splicing junctions. 207955 known splicing junctions. 315939 novel splicing junctions.
sampling 55% (173022386) splicing reads. 537599 splicing junctions. 209091 known splicing junctions. 328508 novel splicing junctions.
sampling 60% (188751694) splicing reads. 550065 splicing junctions. 210018 known splicing junctions. 340047 novel splicing junctions.
sampling 65% (204481002) splicing reads. 561102 splicing junctions. 210818 known splicing junctions. 350284 novel splicing junctions.
sampling 70% (220210309) splicing reads. 570809 splicing junctions. 211526 known splicing junctions. 359283 novel splicing junctions.
sampling 75% (235939617) splicing reads. 579465 splicing junctions. 212157 known splicing junctions. 367308 novel splicing junctions.
sampling 80% (251668925) splicing reads. 586992 splicing junctions. 212677 known splicing junctions. 374315 novel splicing junctions.
sampling 85% (267398233) splicing reads. 593280 splicing junctions. 213113 known splicing junctions. 380167 novel splicing junctions.
sampling 90% (283127541) splicing reads. 598810 splicing junctions. 213504 known splicing junctions. 385306 novel splicing junctions.
sampling 95% (298856849) splicing reads. 603401 splicing junctions. 213833 known splicing junctions. 389568 novel splicing junctions.
sampling 100% (314586157) splicing reads. 607162 splicing junctions. 214074 known splicing junctions. 393088 novel splicing junctions.