Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/82d3b82ba79d285fb61633dd0fb778/A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/f789d11d3dfd58975ba1c1dd754edc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e2/2c87f46248ecfa408627cddc159423/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e2/2c87f46248ecfa408627cddc159423/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/3d013309291690d8fb86b67138cdef/A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15210281) splicing reads. 244314 splicing junctions. 167327 known splicing junctions. 76987 novel splicing junctions.
sampling 10% (30420562) splicing reads. 303494 splicing junctions. 180281 known splicing junctions. 123213 novel splicing junctions.
sampling 15% (45630844) splicing reads. 346793 splicing junctions. 187351 known splicing junctions. 159442 novel splicing junctions.
sampling 20% (60841125) splicing reads. 381818 splicing junctions. 192174 known splicing junctions. 189644 novel splicing junctions.
sampling 25% (76051407) splicing reads. 410501 splicing junctions. 195704 known splicing junctions. 214797 novel splicing junctions.
sampling 30% (91261688) splicing reads. 435201 splicing junctions. 198489 known splicing junctions. 236712 novel splicing junctions.
sampling 35% (106471969) splicing reads. 456370 splicing junctions. 200765 known splicing junctions. 255605 novel splicing junctions.
sampling 40% (121682251) splicing reads. 474972 splicing junctions. 202598 known splicing junctions. 272374 novel splicing junctions.
sampling 45% (136892532) splicing reads. 491229 splicing junctions. 204083 known splicing junctions. 287146 novel splicing junctions.
sampling 50% (152102814) splicing reads. 505690 splicing junctions. 205365 known splicing junctions. 300325 novel splicing junctions.
sampling 55% (167313095) splicing reads. 518352 splicing junctions. 206372 known splicing junctions. 311980 novel splicing junctions.
sampling 60% (182523376) splicing reads. 529789 splicing junctions. 207335 known splicing junctions. 322454 novel splicing junctions.
sampling 65% (197733658) splicing reads. 539953 splicing junctions. 208119 known splicing junctions. 331834 novel splicing junctions.
sampling 70% (212943939) splicing reads. 548660 splicing junctions. 208793 known splicing junctions. 339867 novel splicing junctions.
sampling 75% (228154221) splicing reads. 556222 splicing junctions. 209372 known splicing junctions. 346850 novel splicing junctions.
sampling 80% (243364502) splicing reads. 562814 splicing junctions. 209837 known splicing junctions. 352977 novel splicing junctions.
sampling 85% (258574783) splicing reads. 568374 splicing junctions. 210268 known splicing junctions. 358106 novel splicing junctions.
sampling 90% (273785065) splicing reads. 573085 splicing junctions. 210640 known splicing junctions. 362445 novel splicing junctions.
sampling 95% (288995346) splicing reads. 576995 splicing junctions. 210902 known splicing junctions. 366093 novel splicing junctions.
sampling 100% (304205628) splicing reads. 580103 splicing junctions. 211127 known splicing junctions. 368976 novel splicing junctions.
null device 
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