[W::hts_idx_load3] The index file is older than the data file: BreastNB_FFPE_L01_RNA_01_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (1131653) splicing reads. 101427 splicing junctions. 96048 known splicing junctions. 5379 novel splicing junctions.
sampling 10% (2263307) splicing reads. 111008 splicing junctions. 103939 known splicing junctions. 7069 novel splicing junctions.
sampling 15% (3394960) splicing reads. 115045 splicing junctions. 106923 known splicing junctions. 8122 novel splicing junctions.
sampling 20% (4526614) splicing reads. 117493 splicing junctions. 108557 known splicing junctions. 8936 novel splicing junctions.
sampling 25% (5658268) splicing reads. 119159 splicing junctions. 109591 known splicing junctions. 9568 novel splicing junctions.
sampling 30% (6789921) splicing reads. 120435 splicing junctions. 110312 known splicing junctions. 10123 novel splicing junctions.
sampling 35% (7921575) splicing reads. 121444 splicing junctions. 110848 known splicing junctions. 10596 novel splicing junctions.
sampling 40% (9053229) splicing reads. 122293 splicing junctions. 111262 known splicing junctions. 11031 novel splicing junctions.
sampling 45% (10184882) splicing reads. 122982 splicing junctions. 111594 known splicing junctions. 11388 novel splicing junctions.
sampling 50% (11316536) splicing reads. 123583 splicing junctions. 111862 known splicing junctions. 11721 novel splicing junctions.
sampling 55% (12448190) splicing reads. 124057 splicing junctions. 112074 known splicing junctions. 11983 novel splicing junctions.
sampling 60% (13579843) splicing reads. 124477 splicing junctions. 112246 known splicing junctions. 12231 novel splicing junctions.
sampling 65% (14711497) splicing reads. 124821 splicing junctions. 112380 known splicing junctions. 12441 novel splicing junctions.
sampling 70% (15843151) splicing reads. 125113 splicing junctions. 112497 known splicing junctions. 12616 novel splicing junctions.
sampling 75% (16974804) splicing reads. 125355 splicing junctions. 112579 known splicing junctions. 12776 novel splicing junctions.
sampling 80% (18106458) splicing reads. 125554 splicing junctions. 112649 known splicing junctions. 12905 novel splicing junctions.
sampling 85% (19238112) splicing reads. 125720 splicing junctions. 112707 known splicing junctions. 13013 novel splicing junctions.
sampling 90% (20369765) splicing reads. 125828 splicing junctions. 112753 known splicing junctions. 13075 novel splicing junctions.
sampling 95% (21501419) splicing reads. 125901 splicing junctions. 112787 known splicing junctions. 13114 novel splicing junctions.
sampling 100% (22633073) splicing reads. 125925 splicing junctions. 112795 known splicing junctions. 13130 novel splicing junctions.