File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/13/c0bbf0cfa54676cc4b654af4bf5482/.command.log
Size
35.8 KB
Attempt
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/82d3b82ba79d285fb61633dd0fb778/A673_FFPE_RNA_0001_B23LG7FLT4_3.SJ.out.tab
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/82d3b82ba79d285fb61633dd0fb778/A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/13/c0bbf0cfa54676cc4b654af4bf5482/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/13/c0bbf0cfa54676cc4b654af4bf5482/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b5/82d3b82ba79d285fb61633dd0fb778/A673_FFPE_RNA_0001_B23LG7FLT4_3.Chimeric.out.junction
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5f/3d013309291690d8fb86b67138cdef/A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (7 inputs)

21:23:25 : INFO : -reading targets list: ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu
-mapping reads to genes

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[9960000], rate=12714893.62/min 21:24:23 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file A673_FFPE_RNA_0001_B23LG7FLT4_3.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns.prelim 
Use of uninitialized value $ctat_genome_lib in concatenation (.) or string at /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl line 18.
-A673_FFPE_RNA_0001_B23LG7FLT4_3.introns: identified 7289 cancer introns
21:24:24 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/annotate_cancer_introns.pl --introns_file A673_FFPE_RNA_0001_B23LG7FLT4_3.introns  --ctat_genome_lib ctat_genome_lib_build_dir  --intron_col 0  > A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns.prelim 
21:24:24 : INFO : Running: touch /tmp/nxf.sVSRoTHnn6/A673_FFPE_RNA_0001_B23LG7FLT4_3.chckpts/prelim_introns.ok
21:24:24 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns.prelim --min_total_reads 5  > A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns 
21:24:24 : INFO : Execution Time = 0.01 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/filter_by_min_total_reads.py --cancer_intron_candidates A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns.prelim --min_total_reads 5  > A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns 
21:24:24 : INFO : Running: touch /tmp/nxf.sVSRoTHnn6/A673_FFPE_RNA_0001_B23LG7FLT4_3.chckpts/introns_filtered.ok
21:24:24 : INFO : -found 3626 cancer introns
21:24:24 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns A673_FFPE_RNA_0001_B23LG7FLT4_3.introns  --cancer_introns A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed A673_FFPE_RNA_0001_B23LG7FLT4_3.introns.for_IGV.bed 
21:24:25 : INFO :  Creating the BED File.
21:24:34 : INFO : Saving Bed File as A673_FFPE_RNA_0001_B23LG7FLT4_3.introns.for_IGV.bed
21:24:34 : INFO : Execution Time = 0.17 minutes. CMD: /usr/local/src/CTAT-SPLICING/util/make_igv_splice_bed.py --all_introns A673_FFPE_RNA_0001_B23LG7FLT4_3.introns  --cancer_introns A673_FFPE_RNA_0001_B23LG7FLT4_3.cancer.introns  --genome_lib_dir ctat_genome_lib_build_dir  --output_bed A673_FFPE_RNA_0001_B23LG7FLT4_3.introns.for_IGV.bed 
21:24:34 : INFO : Running: touch /tmp/nxf.sVSRoTHnn6/A673_FFPE_RNA_0001_B23LG7FLT4_3.chckpts/intron_igv_bed.ok
21:24:34 : INFO : Running: /usr/local/src/CTAT-SPLICING/util/igv_read_alignment_extractor.py --igv_introns_bed A673_FFPE_RNA_0001_B23LG7FLT4_3.introns.for_IGV.bed  --bam A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam  --output_prefix A673_FFPE_RNA_0001_B23LG7FLT4_3 
[W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_3.Aligned.sortedByCoord.out.bam.bai