Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/17bf8ace65d43941c87253450d79d0/BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_2.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e5/2f6a869cbdb966c3ae0b76890401e9/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/c4515bbdeed07cfb548c8478389ad2/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e5/2f6a869cbdb966c3ae0b76890401e9/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/17bf8ace65d43941c87253450d79d0/BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_1.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_1.fastp.fastq.gz BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... [progress] 1M reads processed (89.3% mapped) [progress] 2M reads processed (89.3% mapped) [progress] 3M reads processed (89.3% mapped) [progress] 4M reads processed (89.2% mapped) [progress] 5M reads processed (89.2% mapped) [progress] 6M reads processed (89.2% mapped) [progress] 7M reads processed (89.2% mapped) [progress] 8M reads processed (89.2% mapped) done [quant] processed 9,022,667 reads, 8,049,284 reads pseudoaligned [quant] estimated average fragment length: 86.6 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,324 rounds