[W::hts_idx_load3] The index file is older than the data file: 22Rv1_FFPE_RNA_0001_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (325652) splicing reads. 77212 splicing junctions. 73978 known splicing junctions. 3234 novel splicing junctions.
sampling 10% (651305) splicing reads. 96459 splicing junctions. 91393 known splicing junctions. 5066 novel splicing junctions.
sampling 15% (976958) splicing reads. 106161 splicing junctions. 99839 known splicing junctions. 6322 novel splicing junctions.
sampling 20% (1302611) splicing reads. 111918 splicing junctions. 104624 known splicing junctions. 7294 novel splicing junctions.
sampling 25% (1628264) splicing reads. 115857 splicing junctions. 107802 known splicing junctions. 8055 novel splicing junctions.
sampling 30% (1953917) splicing reads. 118745 splicing junctions. 110032 known splicing junctions. 8713 novel splicing junctions.
sampling 35% (2279570) splicing reads. 120922 splicing junctions. 111733 known splicing junctions. 9189 novel splicing junctions.
sampling 40% (2605223) splicing reads. 122690 splicing junctions. 113069 known splicing junctions. 9621 novel splicing junctions.
sampling 45% (2930876) splicing reads. 124080 splicing junctions. 114067 known splicing junctions. 10013 novel splicing junctions.
sampling 50% (3256529) splicing reads. 125176 splicing junctions. 114878 known splicing junctions. 10298 novel splicing junctions.
sampling 55% (3582181) splicing reads. 126068 splicing junctions. 115534 known splicing junctions. 10534 novel splicing junctions.
sampling 60% (3907834) splicing reads. 126828 splicing junctions. 116079 known splicing junctions. 10749 novel splicing junctions.
sampling 65% (4233487) splicing reads. 127450 splicing junctions. 116506 known splicing junctions. 10944 novel splicing junctions.
sampling 70% (4559140) splicing reads. 127915 splicing junctions. 116814 known splicing junctions. 11101 novel splicing junctions.
sampling 75% (4884793) splicing reads. 128333 splicing junctions. 117105 known splicing junctions. 11228 novel splicing junctions.
sampling 80% (5210446) splicing reads. 128644 splicing junctions. 117319 known splicing junctions. 11325 novel splicing junctions.
sampling 85% (5536099) splicing reads. 128914 splicing junctions. 117500 known splicing junctions. 11414 novel splicing junctions.
sampling 90% (5861752) splicing reads. 129128 splicing junctions. 117641 known splicing junctions. 11487 novel splicing junctions.
sampling 95% (6187405) splicing reads. 129262 splicing junctions. 117723 known splicing junctions. 11539 novel splicing junctions.
sampling 100% (6513058) splicing reads. 129311 splicing junctions. 117756 known splicing junctions. 11555 novel splicing junctions.