[W::hts_idx_load3] The index file is older than the data file: 22Rv1_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (341067) splicing reads. 77976 splicing junctions. 74675 known splicing junctions. 3301 novel splicing junctions.
sampling 10% (682134) splicing reads. 96799 splicing junctions. 91630 known splicing junctions. 5169 novel splicing junctions.
sampling 15% (1023202) splicing reads. 105911 splicing junctions. 99527 known splicing junctions. 6384 novel splicing junctions.
sampling 20% (1364269) splicing reads. 111417 splicing junctions. 104085 known splicing junctions. 7332 novel splicing junctions.
sampling 25% (1705336) splicing reads. 115039 splicing junctions. 106988 known splicing junctions. 8051 novel splicing junctions.
sampling 30% (2046404) splicing reads. 117633 splicing junctions. 109019 known splicing junctions. 8614 novel splicing junctions.
sampling 35% (2387471) splicing reads. 119685 splicing junctions. 110574 known splicing junctions. 9111 novel splicing junctions.
sampling 40% (2728538) splicing reads. 121280 splicing junctions. 111772 known splicing junctions. 9508 novel splicing junctions.
sampling 45% (3069606) splicing reads. 122564 splicing junctions. 112719 known splicing junctions. 9845 novel splicing junctions.
sampling 50% (3410673) splicing reads. 123581 splicing junctions. 113442 known splicing junctions. 10139 novel splicing junctions.
sampling 55% (3751740) splicing reads. 124404 splicing junctions. 114020 known splicing junctions. 10384 novel splicing junctions.
sampling 60% (4092808) splicing reads. 125091 splicing junctions. 114485 known splicing junctions. 10606 novel splicing junctions.
sampling 65% (4433875) splicing reads. 125725 splicing junctions. 114904 known splicing junctions. 10821 novel splicing junctions.
sampling 70% (4774942) splicing reads. 126211 splicing junctions. 115228 known splicing junctions. 10983 novel splicing junctions.
sampling 75% (5116010) splicing reads. 126600 splicing junctions. 115493 known splicing junctions. 11107 novel splicing junctions.
sampling 80% (5457077) splicing reads. 126912 splicing junctions. 115709 known splicing junctions. 11203 novel splicing junctions.
sampling 85% (5798144) splicing reads. 127159 splicing junctions. 115866 known splicing junctions. 11293 novel splicing junctions.
sampling 90% (6139212) splicing reads. 127334 splicing junctions. 115974 known splicing junctions. 11360 novel splicing junctions.
sampling 95% (6480279) splicing reads. 127473 splicing junctions. 116067 known splicing junctions. 11406 novel splicing junctions.
sampling 100% (6821347) splicing reads. 127514 splicing junctions. 116090 known splicing junctions. 11424 novel splicing junctions.