Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/441addf83e129f6e17dd56bd065703/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/c4515bbdeed07cfb548c8478389ad2/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/441addf83e129f6e17dd56bd065703/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/39/9bd1b707fab6651f826da940419f0d/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/39/9bd1b707fab6651f826da940419f0d/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: A549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz A549_FFPE_01_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... done [quant] processed 315,474 reads, 7,143 reads pseudoaligned [quant] estimated average fragment length: 38.2045 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 514 rounds