+ mkdir 786-O_FFPE_RNA_0001_B23LG7FLT4_1 + all_qc -bam 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 786-O_FFPE_RNA_0001_B23LG7FLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai 101255it [00:00, 1012456.82it/s] 210670it [00:00, 1060345.35it/s] 317565it [00:00, 1064242.03it/s] 427902it [00:00, 1079661.02it/s] 537088it [00:00, 1084037.77it/s] 645492it [00:00, 1080669.03it/s] 753942it [00:00, 1081899.78it/s] 862134it [00:00, 1079778.79it/s] 972539it [00:00, 1087266.29it/s] 1081269it [00:01, 1073647.55it/s] 1188671it [00:01, 1054779.85it/s] 1297962it [00:01, 1066134.77it/s] 1408537it [00:01, 1077945.58it/s] 1520916it [00:01, 1091627.68it/s] 1631412it [00:01, 1095597.33it/s] 1743599it [00:01, 1102364.01it/s] 1853870it [00:01, 1094310.72it/s] 1965328it [00:01, 1100330.56it/s] 2077653it [00:01, 1107150.79it/s] 2188393it [00:02, 1094828.60it/s] 2317521it [00:02, 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1194984.68it/s] 10555340it [00:08, 1173864.67it/s] 10672995it [00:08, 1147073.22it/s] 10787876it [00:08, 1137327.85it/s] 10901889it [00:08, 1138070.40it/s] 11015758it [00:08, 1129926.66it/s] 11128779it [00:08, 1128569.79it/s] 11241653it [00:08, 1122949.90it/s] 11356904it [00:08, 1131627.09it/s] 11471362it [00:09, 1135432.76it/s] 11584925it [00:09, 1135137.80it/s] 11698453it [00:09, 1131753.86it/s] 11811638it [00:09, 1126130.47it/s] 11925406it [00:09, 1129475.52it/s] 12038363it [00:09, 1126478.73it/s] 12151326it [00:09, 1127286.52it/s] 12265388it [00:09, 1131236.69it/s] 12379837it [00:09, 1135175.88it/s] 12493991it [00:09, 1137055.39it/s] 12607937it [00:10, 1137752.44it/s] 12721715it [00:10, 1137241.81it/s] 12835442it [00:10, 1130049.30it/s] 12948459it [00:10, 1125755.30it/s] 13061434it [00:10, 1126923.23it/s] 13174134it [00:10, 1119735.36it/s] 13286972it [00:10, 1122282.29it/s] 13399412it [00:10, 1122891.36it/s] 13512311it [00:10, 1124687.94it/s] 13625309it [00:10, 1126247.57it/s] 13738643it [00:11, 1128291.89it/s] 13851476it [00:11, 1125755.41it/s] 13964056it [00:11, 1125499.28it/s] 14078036it [00:11, 1129758.09it/s] 14191651it [00:11, 1131646.68it/s] 14304818it [00:11, 1124414.84it/s] 14417271it [00:11, 1123856.96it/s] 14530990it [00:11, 1127697.42it/s] 14644013it [00:11, 1128432.79it/s] 14756862it [00:11, 1125844.08it/s] 14871313it [00:12, 1131331.14it/s] 14985183it [00:12, 1133511.39it/s] 15098538it [00:12, 1131417.63it/s] 15211683it [00:12, 1130938.61it/s] 15324780it [00:12, 1129900.60it/s] 15437772it [00:12, 1128827.01it/s] 15550656it [00:12, 1127018.86it/s] 15663359it [00:12, 1126664.03it/s] 15741054it [00:12, 1224734.14it/s] [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 786-O_FFPE_RNA_0001_B23LG7FLT4_1.md.bai + merge_qc -folders 786-O_FFPE_RNA_0001_B23LG7FLT4_1/786-O_FFPE_RNA_0001_B23LG7FLT4_1.md -output 786-O_FFPE_RNA_0001_B23LG7FLT4_1/786-O_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv + head -n1 786-O_FFPE_RNA_0001_B23LG7FLT4_1/786-O_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 786-O_FFPE_RNA_0001_B23LG7FLT4_1/786-O_FFPE_RNA_0001_B23LG7FLT4_1_full_basic_stats.tsv