=== DEBUGGING INFORMATION ===
Current working directory: /tmp/nxf.rGyNiodMNW
Input files:
  - starfusion_tsv: MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.tsv
  - blacklist: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  - filter_config: starfusion_filter_config.yaml
  - aih_blacklist_breakpoints: blacklist_breakpoint_database_2026-04-27.tsv

=== PYTHON INFORMATION ===
/usr/local/bin/python
Python 3.11.14
/usr/local/bin/python3
Python 3.11.14

=== SCRIPT LOCATION ===
Checking for filter_starfusion_main.py:
/tmp/nxf.rGyNiodMNW/nextflow-bin/filter_starfusion_main.py
filter_starfusion_main.py not in current directory
bin/filter_starfusion_main.py not found
bin/ directory does not exist

=== ENVIRONMENT PATH ===
/tmp/nxf.rGyNiodMNW/nextflow-bin:/usr/local/bin:/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin:/sbin:/bin

=== CURRENT DIRECTORY CONTENTS ===
total 66008
drwx------. 3 1000 root     4096 May 28 22:40 .
drwxrwxrwx. 4 root root       86 May 28 22:40 ..
-rw-r--r--. 1 1000 root        0 May 28 22:40 .command.err
-rw-r--r--. 1 1000 root      882 May 28 22:40 .command.out
-rw-r--r--. 1 1000 root    14581 May 28 22:40 .command.run
-rw-r--r--. 1 1000 root     3224 May 28 22:40 .command.sh
-rw-r--r--. 1 1000 root        0 May 28 22:40 .command.trace
-rw-r--r--. 1 1000 root  2053753 May 28 22:40 MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.tsv
-rw-r--r--. 1 1000 root 10714998 May 28 22:40 blacklist_breakpoint_database_2026-04-27.tsv
-rw-r--r--. 1 1000 root 54779787 May 28 22:40 blacklist_hg38_GRCh38_v2.4.0.tsv.gz
drwxr-xr-x. 4 1000 root     4096 May 28 22:40 nextflow-bin
-rw-r--r--. 1 1000 root     4132 May 28 22:40 starfusion_filter_config.yaml

=== PROJECT DIRECTORY INFO ===
NXF_PROJECT_DIR: not set
baseDir: not set

=== Running filter script ===

================================================================================
STEP 1: LOADING STAR-FUSION PREDICTIONS
================================================================================
Loading STAR-Fusion predictions from: MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.tsv
  ✓ Loaded: 1937 fusions

================================================================================
STEP 2: CONVERTING TO BREAKPOINT_ID FORMAT
================================================================================
✓ Converted to breakpoint_ID format: 1937 valid, 0 invalid

================================================================================
STEP 3: FLAGGING WITH BLACKLIST
================================================================================
Loading blacklist (this may take a while)...
Loading blacklist from: blacklist_hg38_GRCh38_v2.4.0.tsv.gz
  Loaded 9381 single rules and 16721785 pair rules
  Indexed blacklist (bin_size=100000)
Flagging fusions with blacklist...
✓ Flagged 210 of 1937 fusions as blacklisted

================================================================================
STEP 3b: AIH CURATED BREAKPOINT BLACKLIST
================================================================================
Loading AIH blacklist table from: blacklist_breakpoint_database_2026-04-27.tsv
  Qualified breakpoint keys (orientations): 1378
  AIH curated blacklist: matched 121 fusion rows (breakpoint_ID in qualified set)

================================================================================
STEP 4: APPLYING YAML-BASED FILTER
================================================================================
✓ Filter initialized from: starfusion_filter_config.yaml
Applying filter criteria (adding CALL column)...
✓ Filter applied: 97 pass (CALL=1), 1840 fail (CALL=0)
  Total fusions: 1937 (all rows retained)
  Added FILTER column: PASS=97, FAIL=1840

✓ Verified: All 256 blacklisted fusions have CALL=0

================================================================================
STEP 5: GENERATING SUMMARY STATISTICS
================================================================================

================================================================================
FILTERING SUMMARY
================================================================================

Total fusions: 1937
  Pass (CALL=1): 97 (5.0%)
  Fail (CALL=0): 1840 (95.0%)

Blacklist Flagging:
  Total blacklisted: 256
  ✓ All 256 blacklisted fusions have CALL=0

Failure Reasons (top 10):
  Read count too low: 1 < 8: 1028
  Excluded SpliceType: INCL_NON_REF_SPLICE: 414
  Blacklisted: 135
  Blacklisted; aih_curated_blacklist score(3): 75
  Read count too low: 2 < 8: 47
  aih_curated_blacklist score(3): 43
  Excluded annotation pattern: HGNC_GENEFAM: 19
  Read count too low: 4 < 8: 17
  Read count too low: 3 < 8: 14
  Excluded annotation pattern: GTEx_recurrent_StarF2019: 12

Breakdown by SpliceType:
  INCL_NON_REF_SPLICE: 4 pass, 786 fail (total: 790)
  ONLY_REF_SPLICE: 93 pass, 1054 fail (total: 1147)

Read Count Statistics:
  Fusions with < 3 total reads: 1645
  Of these, 1645 have CALL=0

================================================================================
STEP 6: EXPORTING RESULTS
================================================================================
✓ Results exported to: MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.filtered.tsv
  Total fusions: 1937
  CALL=1 (pass): 97
  CALL=0 (fail): 1840
✓ Passing fusions (CALL=1) exported to: MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.filtered_pass_only.tsv
✓ Summary exported to: MCF7_FFPE_RNA_0001_B23LG7FLT4_2.starfusion.starfusion.fusion_predictions.filter_summary.txt

================================================================================
✓ FILTERING COMPLETE
================================================================================