[2026-05-28T20:07:56] Launching Arriba 2.4.0
[2026-05-28T20:07:56] Loading assembly from 'ref_genome.fa' 
[2026-05-28T20:08:11] Loading annotation from 'ref_annot.gtf' 
[2026-05-28T20:08:16] Reading chimeric alignments from 'NTC_0001_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam' (total=66154)
[2026-05-28T20:08:17] Marking multi-mapping alignments (marked=60228)
[2026-05-28T20:08:17] Detecting strandedness (no)
[2026-05-28T20:08:17] Annotating alignments 
[2026-05-28T20:08:18] Filtering duplicates (remaining=36986)
[2026-05-28T20:08:18] Filtering mates which do not map to interesting contigs (1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y AC_* NC_*) (remaining=32038)
[2026-05-28T20:08:18] Filtering mates which only map to viral contigs (AC_* NC_*) (remaining=32038)
[2026-05-28T20:08:18] Filtering viral contigs with expression lower than the top 5 (remaining=32038)
[2026-05-28T20:08:18] Filtering viral contigs with less than 5% coverage (remaining=32038)
[2026-05-28T20:08:18] Estimating fragment length (mate gap mean=-16.2106, mate gap stddev=1.95836, read length mean=46.6676)
[2026-05-28T20:08:18] Filtering read-through fragments with a distance <=10000bp (remaining=32028)
[2026-05-28T20:08:18] Filtering inconsistently clipped mates (remaining=31053)
[2026-05-28T20:08:18] Filtering breakpoints adjacent to homopolymers >=6nt (remaining=29741)
[2026-05-28T20:08:18] Filtering fragments with small insert size (remaining=29611)
[2026-05-28T20:08:18] Filtering alignments with long gaps (remaining=29611)
[2026-05-28T20:08:18] Filtering fragments with both mates in the same gene (remaining=29531)
[2026-05-28T20:08:18] Filtering fusions arising from hairpin structures (remaining=28775)
[2026-05-28T20:08:18] Filtering reads with a mismatch p-value <=0.01 (remaining=410)
[2026-05-28T20:08:18] Filtering reads with low entropy (k-mer content >=60%) (remaining=109)
[2026-05-28T20:08:18] Finding fusions and counting supporting reads (total=119)
[2026-05-28T20:08:18] Merging adjacent fusion breakpoints (remaining=119)
[2026-05-28T20:08:18] Filtering multi-mapping fusions by alignment score and read support (remaining=102)
[2026-05-28T20:08:18] Estimating expected number of fusions by random chance (e-value) 
[2026-05-28T20:08:18] Filtering fusions with both breakpoints in adjacent non-coding/intergenic regions (remaining=100)
[2026-05-28T20:08:18] Filtering intragenic fusions with both breakpoints in exonic regions (remaining=100)
[2026-05-28T20:08:18] Filtering fusions with <2 supporting reads (remaining=3)
[2026-05-28T20:08:18] Filtering fusions with an e-value >=0.3 (remaining=3)
[2026-05-28T20:08:18] Searching for internal tandem duplications <=100bp with >=10 supporting reads and >=7% allele fraction (remaining=3)
[2026-05-28T20:08:18] Filtering fusions with both breakpoints in intronic/intergenic regions (remaining=0)
[2026-05-28T20:08:18] Searching for known fusions in 'known_fusions_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=0)
[2026-05-28T20:08:18] Filtering in vitro-generated fusions between genes with an expression above the 99.8% quantile (remaining=0)
[2026-05-28T20:08:19] Searching for fusions with spliced split reads (remaining=0)
[2026-05-28T20:08:19] Selecting best breakpoints from genes with multiple breakpoints (remaining=0)
[2026-05-28T20:08:19] Filtering read-through fusions with breakpoints near the gene boundary (remaining=0)
[2026-05-28T20:08:19] Searching for fusions with >=4 spliced events (remaining=0)
[2026-05-28T20:08:19] Filtering blacklisted fusions in 'blacklist_hg38_GRCh38_v2.4.0.tsv.gz' (remaining=0)
[2026-05-28T20:08:34] Filtering fusions with anchors <=23nt (remaining=0)
[2026-05-28T20:08:34] Filtering end-to-end fusions with low support (remaining=0)
[2026-05-28T20:08:34] Filtering fusions with no coverage around the breakpoints (remaining=0)
[2026-05-28T20:08:34] Indexing gene sequences 
[2026-05-28T20:08:34] Filtering genes with >=30% identity (remaining=0)
[2026-05-28T20:08:34] Re-aligning chimeric reads to filter fusions with >=80% mis-mappers (remaining=0)
[2026-05-28T20:08:34] Selecting best breakpoints from genes with multiple breakpoints (remaining=0)
[2026-05-28T20:08:34] Searching for additional isoforms (remaining=0)
[2026-05-28T20:08:34] Assigning confidence scores to events 
[2026-05-28T20:08:34] Loading protein domains from 'protein_domains_hg38_GRCh38_v2.4.0.gff3'
[2026-05-28T20:08:35] Writing fusions to file 'NTC_0001_0001_B23LG7FLT4_1.arriba.fusions.tsv' 
[2026-05-28T20:08:35] Writing discarded fusions to file 'NTC_0001_0001_B23LG7FLT4_1.arriba.fusions.discarded.tsv'
[2026-05-28T20:08:35] Freeing resources
[2026-05-28T20:08:36] Done (elapsed time=00:00:40, CPU time=00:00:38, peak memory=4.14gb)