Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/f789d11d3dfd58975ba1c1dd754edc/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/007c00020155c7ced109db46829728/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e4/f8427caf11ff08063a616c85722d32/A673_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/007c00020155c7ced109db46829728/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/d9/ad42f26f08b5f2674c6fc28c64147a/A673_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: A673_FFPE_RNA_0001_B23LG7FLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15771963) splicing reads. 251040 splicing junctions. 168853 known splicing junctions. 82187 novel splicing junctions.
sampling 10% (31543926) splicing reads. 314679 splicing junctions. 181890 known splicing junctions. 132789 novel splicing junctions.
sampling 15% (47315889) splicing reads. 361613 splicing junctions. 188989 known splicing junctions. 172624 novel splicing junctions.
sampling 20% (63087852) splicing reads. 399404 splicing junctions. 193925 known splicing junctions. 205479 novel splicing junctions.
sampling 25% (78859815) splicing reads. 431509 splicing junctions. 197559 known splicing junctions. 233950 novel splicing junctions.
sampling 30% (94631778) splicing reads. 458813 splicing junctions. 200439 known splicing junctions. 258374 novel splicing junctions.
sampling 35% (110403742) splicing reads. 482550 splicing junctions. 202771 known splicing junctions. 279779 novel splicing junctions.
sampling 40% (126175705) splicing reads. 503182 splicing junctions. 204620 known splicing junctions. 298562 novel splicing junctions.
sampling 45% (141947668) splicing reads. 521646 splicing junctions. 206157 known splicing junctions. 315489 novel splicing junctions.
sampling 50% (157719631) splicing reads. 538397 splicing junctions. 207483 known splicing junctions. 330914 novel splicing junctions.
sampling 55% (173491594) splicing reads. 552896 splicing junctions. 208588 known splicing junctions. 344308 novel splicing junctions.
sampling 60% (189263557) splicing reads. 566034 splicing junctions. 209551 known splicing junctions. 356483 novel splicing junctions.
sampling 65% (205035520) splicing reads. 577572 splicing junctions. 210330 known splicing junctions. 367242 novel splicing junctions.
sampling 70% (220807484) splicing reads. 587743 splicing junctions. 211018 known splicing junctions. 376725 novel splicing junctions.
sampling 75% (236579447) splicing reads. 596783 splicing junctions. 211676 known splicing junctions. 385107 novel splicing junctions.
sampling 80% (252351410) splicing reads. 604673 splicing junctions. 212233 known splicing junctions. 392440 novel splicing junctions.
sampling 85% (268123373) splicing reads. 611479 splicing junctions. 212693 known splicing junctions. 398786 novel splicing junctions.
sampling 90% (283895336) splicing reads. 617289 splicing junctions. 213065 known splicing junctions. 404224 novel splicing junctions.
sampling 95% (299667299) splicing reads. 622165 splicing junctions. 213373 known splicing junctions. 408792 novel splicing junctions.
sampling 100% (315439263) splicing reads. 626028 splicing junctions. 213632 known splicing junctions. 412396 novel splicing junctions.
null device 
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