Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/aafd8ee649097fec8815493f74879b/22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_2.fastp.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/66/c6f6d610d08093d567faca6752667c/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/aafd8ee649097fec8815493f74879b/22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_1.fastp.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/c4515bbdeed07cfb548c8478389ad2/kallisto
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/66/c6f6d610d08093d567faca6752667c/.command.run
==> STAGING COMPLETE (6 inputs)


[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 194,360
[index] number of k-mers: 112,035,259
[quant] running in paired-end mode
[quant] will process pair 1: 22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_1.fastp.fastq.gz
                             22Rv1_FFPE_RNA_0001_B23LG7FLT4_3_2.fastp.fastq.gz
[quant] finding pseudoalignments for the reads ...
[progress] 1M reads processed (84.6% mapped)             
[progress] 2M reads processed (84.6% mapped)             
[progress] 3M reads processed (84.6% mapped)             
[progress] 4M reads processed (84.6% mapped)             
[progress] 5M reads processed (84.6% mapped)             
[progress] 6M reads processed (84.6% mapped)             
[progress] 7M reads processed (84.6% mapped)             
[progress] 8M reads processed (84.6% mapped)             
[progress] 9M reads processed (84.6% mapped)             
[progress] 10M reads processed (84.6% mapped)             
[progress] 11M reads processed (84.6% mapped)             
[progress] 12M reads processed (84.6% mapped)             
[progress] 13M reads processed (84.6% mapped)              done
[quant] processed 13,874,867 reads, 11,737,431 reads pseudoaligned
[quant] estimated average fragment length: 102.6
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,138 rounds