Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/39/9bd1b707fab6651f826da940419f0d/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/9b7e7b2ef1237e3e9faba4ba632e7a/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/9b7e7b2ef1237e3e9faba4ba632e7a/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/39/9bd1b707fab6651f826da940419f0d/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_1.fastp.fastq.gz input2/A549_FFPE_01_RNA_0001_B23LG7FLT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix A549_FFPE_01_RNA_0001_B23LG7FLT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:A549_FFPE_01_RNA_0001_B23LG7FLT4_1 SM:A549_FFPE_01_RNA_0001_B23LG7FLT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source May 28 20:02:58 ..... started STAR run May 28 20:02:58 ..... loading genome May 28 20:03:12 ..... processing annotations GTF May 28 20:03:22 ..... inserting junctions into the genome indices May 28 20:04:31 ..... started mapping May 28 20:05:04 ..... finished mapping May 28 20:05:05 ..... started sorting BAM May 28 20:05:06 ..... finished successfully