#!/bin/bash -euo pipefail python -u /usr/local/src/CTAT-SPLICING/STAR_to_cancer_introns.py \ --SJ_tab_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.SJ.out.tab \ --chimJ_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.Chimeric.out.junction \ --bam_file BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.Aligned.sortedByCoord.out.bam \ --output_prefix BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2 \ --ctat_genome_lib ctat_genome_lib_build_dir \ --vis --sample_name BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2 # Sort output files to ensure consistent ordering across runs while preserving headers if [ -f BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns ]; then # Extract header (first line) and sort data lines by fourth column (uniq_mapped) descending, then by first column (intron) head -n 1 BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns > BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns.tmp tail -n +2 BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns | LC_ALL=C sort -k4,4nr -k1,1 >> BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns.tmp mv BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns.tmp BreastNP_FFPE_L02_RNA_01_B23LG7FLT4_2.cancer.introns fi cat <<-END_VERSIONS > versions.yml "NFCORE_RNAFUSION:RNAFUSION:CTATSPLICING_STARTOCANCERINTRONS": ctat-splicing: 0.0.3 END_VERSIONS