Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/4e557baa29838aaa3a615aa612220e/A549_FFPE_01_RNA_0001_B23LG7FLT4_3_2.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/e071fc1e1be540aba9cf3090aa15ec/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/55/c4515bbdeed07cfb548c8478389ad2/kallisto Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/e071fc1e1be540aba9cf3090aa15ec/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/01/4e557baa29838aaa3a615aa612220e/A549_FFPE_01_RNA_0001_B23LG7FLT4_3_1.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 194,360 [index] number of k-mers: 112,035,259 [quant] running in paired-end mode [quant] will process pair 1: A549_FFPE_01_RNA_0001_B23LG7FLT4_3_1.fastp.fastq.gz A549_FFPE_01_RNA_0001_B23LG7FLT4_3_2.fastp.fastq.gz [quant] finding pseudoalignments for the reads ... done [quant] processed 393,174 reads, 5,736 reads pseudoaligned [quant] estimated average fragment length: 35.1978 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 646 rounds